Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Instructions
Code CSB-E13027r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name fatty acid binding protein 4, adipocyte
Alternative Names A-FABP, aP2,
Abbreviation FABP4
Uniprot No. P70623
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %88-100
1:2Average %85
Range %80-90
1:4Average %89
Range %84-99
1:8Average %92
Range %86-101
Recovery
The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9082-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1001.974 1.913 1.944 1.817
501.721 1.681 1.701 1.574
251.416 1.311 1.364 1.237
12.50.958 0.897 0.928 0.801
6.250.669 0.629 0.649 0.522
3.120.395 0.364 0.380 0.253
1.560.217 0.207 0.212 0.085
00.131 0.123 0.127  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
Subcellular Location Cytoplasm, Nucleus
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

STRING: 10116.ENSRNOP00000014701

UniGene: Rn.4258

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