Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Code CSB-E13027r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P70623
Lead Time 3-5 working days
Abbreviation FABP4
Protein Biological Process 1 Transport
Target Name fatty acid binding protein 4, adipocyte
Alias A-FABP, aP2,
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
HGNC 3559
RGD 69309
MGI 88038
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %88-100
1:2Average %85
Range %80-90
1:4Average %89
Range %84-99
1:8Average %92
Range %86-101
Recovery
The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9082-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1001.974 1.913 1.944 1.817
501.721 1.681 1.701 1.574
251.416 1.311 1.364 1.237
12.50.958 0.897 0.928 0.801
6.250.669 0.629 0.649 0.522
3.120.395 0.364 0.380 0.253
1.560.217 0.207 0.212 0.085
00.131 0.123 0.127  
References
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Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus (By similarity).
Subcellular Location Cytoplasm, Nucleus
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

STRING: 10116.ENSRNOP00000014701

UniGene: Rn.4258

Pathway PPAR signaling pathway

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