Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Code CSB-E13027r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The product CSB-E13027r is a sandwich ELISA kit developed to measure concentrations of rat FABP4 in serum, plasma, cell lysates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FABP4 in samples. The color intensity is measured at 450 nm via a microplate reader.

FABP4, also known as A-FABP or aP2, is mainly expressed in adipocytes and functions as a lipid chaperone protein. It regulates lipolysis and adipogenesis through interactions with hormone-sensitive lipase (HSL) and peroxisome PPAR-γ, respectively. FABP4 plays an important role in the development of insulin resistance and atherosclerosis in association with metaflammation. An increase in circulating FABP4 concentrations is linked to obesity, insulin resistance, diabetes, hypertension, cardiac dysfunction, and atherosclerosis. Reducing FABP4 levels or activity is associated with improved metabolic health.

Target Name fatty acid binding protein 4, adipocyte
Alternative Names
Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit
Abbreviation FABP4
Uniprot No. P70623
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %93
Range %88-100
1:2Average %85
Range %80-90
1:4Average %89
Range %84-99
1:8Average %92
Range %86-101
The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9082-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1001.974 1.913 1.944 1.817
501.721 1.681 1.701 1.574
251.416 1.311 1.364 1.237
12.50.958 0.897 0.928 0.801
6.250.669 0.629 0.649 0.522
3.120.395 0.364 0.380 0.253
1.560.217 0.207 0.212 0.085
00.131 0.123 0.127  
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Subcellular Location Cytoplasm. Nucleus.
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Database Links

STRING: 10116.ENSRNOP00000014701

UniGene: Rn.4258

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