Rat fibroblast growth factor 21 (FGF21) ELISA kit

Code CSB-EL008627RA
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation FGF21
Target Name fibroblast growth factor 21
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %98
Range %94-102
1:2Average %91
Range %86-97
1:4Average %106
Range %102-114
1:8Average %95
Range %90-100
The recovery of rat FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-90
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
15002.646 2.772 2.709 2.590
7501.973 1.932 1.953 1.834
3751.217 1.302 1.260 1.141
187.50.789 0.793 0.791 0.672
940.463 0.449 0.456 0.337
470.302 0.281 0.292 0.173
23.50.195 0.205 0.200 0.081
00.121 0.117 0.119
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