Rat growth differentiation factor 15(GDF15)ELISA Kit

Instructions
Code CSB-E16317r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth differentiation factor 15
Alternative Names Gdf15 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit
Abbreviation GDF15
Uniprot No. Q9Z0J6
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 92  
Range % 87-96  
1:20 Average % 83  
Range % 80-86  
1:40 Average % 96  
Range % 93-99  
1:80 Average % 102  
Range % 99-105  
Recovery
The recovery of rat GDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-90  
EDTA plasma (n=4) 93 89-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.127 0.129 0.128    
50 0.208 0.220 0.214    
25 0.334 0.324 0.329    
12.5 0.472 0.498 0.485    
6.25 0.713 0.735 0.724    
3.12 1.162 1.139 1.151    
1.56 1.542 1.567 1.555    
0 2.814 2.864 2.839    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Gene References into Functions
  1. These findings demonstrate the importance of the AP in the mediation of cancer-dependent anorexia and body weight loss and support a pathological role of MIC-1 as a tumour-derived factor mediating CACS, possibly via an AP-dependent action. PMID: 28025863
  2. Plasma GDF-15 was increased 3, 12 and 26, while plasma TGF-beta was increased 12 weeks after irradiation. PMID: 27566082
  3. the time course of the expression of growth differentiation factor15 (GDF15) in rat ischemic myocardium with increasing durations of reperfusion, and to elucidate its physiopathological role in the noreflow phenomenon, is reported. PMID: 26647773
  4. findings indicate that the attenuation of REV-ERBalpha leads to an upregulation of Gdf10 and Gdf15 in decidual cells PMID: 26811051
  5. GDF15 protects the renal interstitium and tubular compartment in experimental type 1 and 2 diabetes without affecting glomerular damage PMID: 23986522
  6. Up-regulation of EGR-1 and NAG-1, along with higher reactive oxygen species load may positively regulate the intrinsic pathway of apoptosis for the NSAID mediated chemoprevention of colorectal cancer. PMID: 23435960
  7. Gdf15 expression exhibits increases >10-fold at night in the rat pineal gland, reaching levels that are >15-fold higher than the median in other tissues. PMID: 19103603

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Subcellular Location Secreted
Protein Families TGF-beta family
Tissue Specificity Detected in plasma (at protein level).
Database Links

KEGG: rno:29455

STRING: 10116.ENSRNOP00000026652

UniGene: Rn.44228

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