Rat growth hormone,GH ELISA KIT

Code CSB-E07342r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
growth hormone 1
Alternative Names
Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
Abbreviation
GH1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %98
Range %94-105
1:200Average %96
Range %90-102
1:400Average %100
Range %94-106
1:800Average %93
Range %89-97
Recovery
The recovery of rat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9691-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.718 2.801 2.760 2.580
1002.346 2.405 2.376 2.196
501.754 1.763 1.759 1.579
251.126 1.157 1.142 0.962
12.50.657 0.668 0.663 0.483
6.250.458 0.467 0.463 0.283
3.120.341 0.358 0.350 0.170
00.179 0.181 0.180  
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat GH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled GH antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat GH1 ELISA Kit was designed for the quantitative measurement of Rat GH1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Target Background

Function
(From Uniprot)
GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
Gene References into Functions
  1. GHRH agonistic analogs, JI-34 and MR-356 alleviated radiation-induced damage of cardiac myocytes. PMID: 27480202
  2. findings support the hypothesis that antagonizing the signaling of GHRH in T1D may improve GLP-1 function in the small intestine, which, in turn, diminishes TRL and reduces renal and vascular complications PMID: 26831066
  3. JI-38 had shown promising results in multiple fields including cardiology, diabetes and wound healing, our new, more potent, GHRH agonists should manifest additional efficacy for possible medical applications. PMID: 24373935
  4. GHRH protects the heart from ischemia/reperfusion injury through receptor-mediated mechanisms, leading to activation of RISK and SAFE pathways, which converge on mitochondria and possibly on AMP-activated protein kinase. PMID: 23417421
  5. We present evidence that growth retardation in upper airway obstruction is associated with a reduction in hypothalamic GHRH content. PMID: 21406516
  6. Growth hormone expression rises in pituitary gland during normal pregnancy, while its receptor mRNA level in hypothalamus decreases. PMID: 20814072
  7. GABAergic neurons in the median preoptic nucleus and ventrolateral preoptic nucleus are potential targets of the sleep-regulatory actions of GHRH. PMID: 19889861
  8. binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). PMID: 11814616
  9. direct interactions between the growth hormone releasing hormone and growth hormone secretagogue receptors may explain the observed effects of of ghrelin and growth hormone secretagogues on signal transduction PMID: 12446584
  10. Review. Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. PMID: 12511850
  11. Changes in ghrelin and ghrelin receptor expression according to feeding status. PMID: 12876464
  12. Growth hormone releasing hormone (GHRH) is involved in the transcriptional regulation of the GH gene. PMID: 14664705
  13. results suggest that growth hormone secretagogue receptor is involved in the up-regulation of growth hormone releasing hormone and neuropeptide Y expression PMID: 16728494
  14. RFRP-3 infusion elicited a significant increase in growth hormone-releasing hormone mRNA and plasma growth hormone levels compared to control rats. PMID: 18628614
  15. Growth hormone-releasing hormone promotes survival of cardiac myocytes in vitro and protects against ischaemia-reperfusion injury in rat heart. PMID: 19293247
  16. Postsynaptic localization of sst(1) receptors and synaptic connectivity may be an important mechanism by which sst(1) mediates SRIF's cyclical effects on ARC GHRH neurons to generate the ultradian rhythm of GH secretion. PMID: 19553459

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Subcellular Location
Secreted.
Protein Families
Glucagon family
Database Links
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