Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Instructions
Code CSB-E07299r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name amphiregulin
Alternative Names Areg ELISA Kit; SdgfAmphiregulin ELISA Kit; AR ELISA Kit; Schwannoma-derived growth factor ELISA Kit; SDGF ELISA Kit
Abbreviation AREG
Uniprot No. P24338
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 pg/mL-80 pg/mL
Sensitivity 0.31 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-98
1:2Average %95
Range %91-99
1:4Average %86
Range %80-92
1:8Average %96
Range %92-102
Recovery
The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10498-108
EDTA plasma (n=4)9792-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
802.538 2.616 2.577 2.413
401.939 2.059 1.999 1.835
201.364 1.386 1.375 1.211
100.882 0.870 0.876 0.712
50.549 0.541 0.545 0.381
2.50.341 0.356 0.349 0.185
1.250.253 0.258 0.256 0.092
00.168 0.160 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Gene References into Functions
  1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
  2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
  3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
  4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
  5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996

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Subcellular Location Membrane, Single-pass membrane protein
Protein Families Amphiregulin family
Database Links

KEGG: rno:29183

STRING: 10116.ENSRNOP00000003703

UniGene: Rn.10568

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