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Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Protocol MSDS
Code CSB-E07299r
Size 96T,5×96T,10×96T
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Product Details

Target Name
amphiregulin
Alternative Names
Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
Abbreviation
AREG
Uniprot No.
P24338
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 pg/mL-80 pg/mL
Sensitivity
0.31 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %92
Range %87-98
1:2Average %95
Range %91-99
1:4Average %86
Range %80-92
1:8Average %96
Range %92-102
Recovery
The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10498-108
EDTA plasma (n=4)9792-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
802.538 2.616 2.577 2.413
401.939 2.059 1.999 1.835
201.364 1.386 1.375 1.211
100.882 0.870 0.876 0.712
50.549 0.541 0.545 0.381
2.50.341 0.356 0.349 0.185
1.250.253 0.258 0.256 0.092
00.168 0.160 0.164 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Rat Keratinocyte Autocrine Factor/Amphiregulin (KAF/AR) ELISA Kit is designed to measure Amphiregulin (AR) in rat serum, plasma, and tissue homogenates with exceptional accuracy and sensitivity.

With a detection range of 1.25 pg/mL-80 pg/mL and a sensitivity of 0.31 pg/mL, this quantitative assay can detect even the smallest amount of KAF/AR in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

This kit is ideal for researchers investigating the role of KAF/AR in cancer、activates intracellular signaling, lung morphogenesis, breast bone tissue and oocyte maturation, and tissue integrity recovery after infection or injury（激活细胞内信号、肺形态发生及乳腺骨组织和卵母细胞的发育成熟、感染或损伤后的组织完整性恢复）. The sandwich measurement principle allows for accurate quantification of KAF/AR in your samples, making it a valuable tool for any cancer research project. With its quick assay time, low sample volume requirement, and precise results, this kit is a must-have for any researcher studying cancer.

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Areg Proteins

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Target Background

Function
(From Uniprot)
Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Gene References into Functions
  1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
  2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
  3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
  4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
  5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996

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Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Amphiregulin family
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