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| Code | CSB-E07299r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 98-108 | ||||
| EDTA plasma (n=4) | 97 | 92-106 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Keratinocyte Autocrine Factor/Amphiregulin (KAF/AR) ELISA Kit is designed to measure Amphiregulin (AR) in rat serum, plasma, and tissue homogenates with exceptional accuracy and sensitivity.
With a detection range of 1.25 pg/mL-80 pg/mL and a sensitivity of 0.31 pg/mL, this quantitative assay can detect even the smallest amount of KAF/AR in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.
This kit is ideal for researchers investigating the role of KAF/AR in cancer、activates intracellular signaling, lung morphogenesis, breast bone tissue and oocyte maturation, and tissue integrity recovery after infection or injury（激活细胞内信号、肺形态发生及乳腺骨组织和卵母细胞的发育成熟、感染或损伤后的组织完整性恢复）. The sandwich measurement principle allows for accurate quantification of KAF/AR in your samples, making it a valuable tool for any cancer research project. With its quick assay time, low sample volume requirement, and precise results, this kit is a must-have for any researcher studying cancer.
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