Rat lipolysaccharide binding protein,LBP ELISA Kit

Code CSB-E11184r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
lipopolysaccharide binding protein
Alternative Names
LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
Abbreviation
LBP
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %85
Range %81-89
1:10Average %94
Range %91-98
1:20Average %91
Range %85-97
1:40Average %86
Range %82-89
Recovery
The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10097-103
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.869 2.899 2.884 2.688
20002.231 2.268 2.250 2.054
10001.514 1.569 1.542 1.346
5000.969 0.999 0.984 0.788
2500.628 0.648 0.638 0.442
1250.432 0.469 0.451 0.255
62.50.297 0.278 0.288 0.092
00.197 0.195 0.196
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat LBP ELISA Kit was designed for the quantitative measurement of Rat LBP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Subcellular Location
Secreted. Cytoplasmic granule membrane.
Protein Families
BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity
Detected in lung, liver, heart and kidney.
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