Rat lipolysaccharide binding protein,LBP ELISA Kit

Instructions
Code CSB-E11184r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipopolysaccharide binding protein
Alternative Names LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
Abbreviation LBP
Uniprot No. Q63313
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %85
Range %81-89
1:10Average %94
Range %91-98
1:20Average %91
Range %85-97
1:40Average %86
Range %82-89
Recovery
The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10097-103
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.869 2.899 2.884 2.688
20002.231 2.268 2.250 2.054
10001.514 1.569 1.542 1.346
5000.969 0.999 0.984 0.788
2500.628 0.648 0.638 0.442
1250.432 0.469 0.451 0.255
62.50.297 0.278 0.288 0.092
00.197 0.195 0.196
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Subcellular Location Secreted, Cytoplasmic granule membrane
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity Detected in lung, liver, heart and kidney.
Database Links

STRING: 10116.ENSRNOP00000019787

UniGene: Rn.48863

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