Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

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Product Details

Alternative Names
Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.235 ng/ml - 15 ng/ml
Sensitivity
0.058 ng/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

89

Range %

85-94

1:2

Average %

92

Range %

87-98

1:4

Average %

95

Range %

90-102

1:8

Average %

100

Range %

96-109

Recovery

The recovery of rat MMP-1/Collagenase I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

84

80-89

EDTA plasma (n=4)

97

90-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

15

2.257

2.231

2.244

2.147

7.5

1.876

1.832

1.854

1.757

3.75

1.381

1.373

1.377

1.280

1.88

0.835

0.797

0.816

0.719

0.94

0.397

0.376

0.387

0.290

0.47

0.273

0.257

0.265

0.168

0.235

0.197

0.207

0.202

0.105

0

0.099

0.094

0.097

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat MMP1 ELISA Kit was designed for the quantitative measurement of Rat MMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.235 ng/ml - 15 ng/ml and the sensitivity is 0.058 ng/ml.

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links

UniGene: Rn.79007

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