Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

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Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

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Citations (7) Protocol MSDS

Code	CSB-E07416r
Size	$615
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Citations (7)
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Alternative Names

Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit

Abbreviation

MMP1

Uniprot No.

P81563

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.235 ng/ml - 15 ng/ml

Sensitivity

0.058 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	92
	Range %	87-98
1:4	Average %	95
	Range %	90-102
1:8	Average %	100
	Range %	96-109

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.257

2.231

2.244

2.147

7.5

1.876

1.832

1.854

1.757

3.75

1.381

1.373

1.377

1.280

1.88

0.835

0.797

0.816

0.719

0.94

0.397

0.376

0.387

0.290

0.47

0.273

0.257

0.265

0.168

0.235

0.197

0.207

0.202

0.105

0.099

0.094

0.097

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat MMP1 ELISA Kit was designed for the quantitative measurement of Rat MMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.235 ng/ml - 15 ng/ml and the sensitivity is 0.058 ng/ml.

Photoaging induced by long-term exposure to UV irradiation and amelioration by Skipjack tuna skin hydrolysates: Targeting inhibition of MAPK and NF-κB signaling hyperactivation D Xu,Journal of Functional Foods,2022
Dietary Supplementation with Palmitoyl-Glucosamine Co-Micronized with Curcumin Relieves Osteoarthritis Pain and Benefits Joint Mobility E Gugliandolo,Animals,2020
Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats G Shamlan,Arabian Journal for Science and Engineering,2020
Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain Cordaro M, et al,Arthritis Research & Therapy,2019
A novel composite formulation of palmitoylethanolamide and quercetinDecreases inflammation and relieves pain in inflammatory and osteoarthritic pain models Britti D.et al,BMC Vet Res.,2017
Adelmidrol, in combination with hyaluronic acid, displays increased anti-inflammatory and analgesic effects against monosodium iodoacetate-induced osteoarthritis in rats Rosanna Di Paola.et al,Arthritis Research & Therapy,2016
Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Peptidase M10A family

Database Links

UniGene: Rn.79007

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Related pathways

PPAR signaling pathway IL-17 signaling pathway

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