Rat neuropeptide Y (NPY) ELISA kit

Instructions
Code CSB-E13431r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name neuropeptide Y
Abbreviation NPY
Uniprot No. P07808
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 92-104  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 93  
Range % 89-95  
1:8 Average % 86  
Range % 82-92  
Recovery
The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-99  
EDTA plasma (n=4) 105 100-110  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.487 2.385 2.436 2.290  
50 1.629 1.527 1.578 1.432  
25 0.916 0.937 0.927 0.781  
12.5 0.574 0.565 0.570 0.424  
6.25 0.375 0.386 0.381 0.235  
3.12 0.261 0.250 0.256 0.110  
1.56 0.186 0.182 0.184 0.038  
0 0.146 0.145 0.146    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Subcellular Location Secreted
Protein Families NPY family
Tissue Specificity One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Database Links

KEGG: rno:100912228

STRING: 10116.ENSRNOP00000013146

UniGene: Rn.9714

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