Rat oxyntomodulin ELISA Kit

Code CSB-E12949r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name oxyntomodulin
Abbreviation OXM
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %81-92
1:2Average %96
Range %90-102
1:4Average %97
Range %92-104
1:8Average %93
Range %90-96
The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-100
EDTA plasma (n=4)9995-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
80002.356 2.378 2.367 2.252
40002.121 2.161 2.141 2.026
20001.757 1.798 1.778 1.663
10001.343 1.391 1.367 1.252
5000.851 0.899 0.875 0.760
2500.502 0.539 0.521 0.406
1250.272 0.291 0.282 0.167
00.114 0.116 0.115  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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