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Human oxyntomodulin ELISA Kit

Citations (5) Protocol MSDS
Code CSB-E12948h
Size 96T,5×96T,10×96T
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Product Details

Target Name
oxyntomodulin
Abbreviation
OXM
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %89
Range %83-94
1:2Average %84
Range %80-89
1:4Average %95
Range %89-99
1:8Average %92
Range %88-96
Recovery
The recovery of human Oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9892-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.263 2.181 2.222 2.042
101.761 1.791 1.776 1.596
51.263 1.201 1.232 1.052
2.50.766 0.723 0.745 0.565
1.250.443 0.417 0.430 0.250
0.6250.274 0.253 0.264 0.084
0.3120.267 0.282 0.275 0.095
00.181 0.179 0.180 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Human oxyntomodulin ELISA Kit is specifically designed for the detection and quantification of oxyntomodulin in human samples. Oxyntomodulin is a hormone produced in the gastrointestinal tract that has been linked to various metabolic functions, making our ELISA kit an essential tool for researchers in the field of Metabolism.

This ELISA kit is optimized for the detection of oxyntomodulin in human serum, plasma, cell culture supernates, urine, and tissue homogenates. With a detection range of 0.312 ng/mL-20 ng/mL and a sensitivity of 0.078 ng/mL, our kit provides highly accurate and reliable results, allowing you to obtain precise measurements of oxyntomodulin in your samples.

The assay time ranges from 1-5 hours, and only requires a sample volume of 50-100ul, making our ELISA kit a quick and efficient solution for your research needs. The detection wavelength of 450 nm provides clear and reliable readings, while over 5 citations demonstrate the scientific validity and reliability of this kit.

Citations

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