Rat prealbumin,PA ELISA Kit

Code CSB-E13251r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
prealbumin,PA
Alternative Names
Ttr ELISA Kit; Tt ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit; TBPA ELISA Kit
Abbreviation
PA
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
375 ng/mL-6000 ng/mL
Sensitivity
375 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 103  
Range % 99-107  
1:800 Average % 98  
Range % 95-103  
1:1600 Average % 93  
Range % 87-97  
Recovery
The recovery of rat PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 95-103  
EDTA plasma (n=4) 89 82-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
6000 0.152 0.163 0.158    
3000 0.288 0.300 0.294    
1500 0.561 0.568 0.565    
750 0.903 0.925 0.914    
375 1.477 1.434 1.456    
0 2.662 2.591 2.627    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat prealbumin (PA) ELISA Kit was designed for the quantitative measurement of Rat prealbumin (PA) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 375 ng/mL-6000 ng/mL and the sensitivity is 375 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Gene References into Functions
  1. Data suggest that the mutated transthyretin (TTR) seems to have potential as biomarker for early detection of hepatocellular carcinoma (HCC). PMID: 28876464
  2. Serum transthyretin level dramatically decreased in animals displaying decompression sickness symptoms. PMID: 27068327
  3. We conclude that stress upregulates TTR expression in choroid plexus. PMID: 22371232
  4. Decreased trophoblast expression of retinol transport proteins suggest that nitrofen may interfere with the fetal retinol transport resulting in reduced pulmonary RBP and TTR levels and causing pulmonary hypoplasia in CDH. PMID: 22015466
  5. Data demonstrate that, in vitro and in vivo, transthyretin is up-regulated by progesterone, and suggest that this response is mediated through a progesterone receptor-mediated mechanism. PMID: 20535645
  6. Structural basis of negative cooperativity in transthyretin PMID: 11995997
  7. Complex of rat transthyretin with tetraiodothyroacetic acid refined at 2.1 and 1.8 A resolution PMID: 11995998
  8. Comparison of binding interactions of dibromoflavonoids with transthyretin PMID: 11995999
  9. Amyloid formation in rat transthyretin: effect of oxidative stress PMID: 12135814
  10. data show the LR1 gene is up-regulated following short interval successive partial hepatectomy; it may play an important role in liver regeneration PMID: 12508371
  11. Data suggest that monomeric transthyretin may represent an ischemia-specific CSF marker to indicate the sequential changes according to ischemic insults of the brain. PMID: 15469881
  12. Our study provides genetic, behavioural and molecular evidence that TTR is involved in the maintenance of normal cognitive processes during aging by acting on the retinoid signalling pathway. PMID: 17512093
  13. Ttr mRNA expression in dorsal root ganglia and cauda equina. PMID: 18406527
  14. Sex hormones may increase TTR synthesis by the liver, and therefore, may contribute to amyloid deposition. PMID: 18568387
  15. Results suggest that transthyretin is up-regulated by estradiol via a pathway involving estrogen receptors alpha and beta. PMID: 19130215
  16. The expression of transthyretin in brain of a transgenic mouse model of Alzheimer's disease is asymmetric and affected by age and sex. PMID: 19167467
  17. rat and murine TTR have a lower intrinsic beta-aggregation propensity and a similar native beta-structure stability compared with human TTR. PMID: 19602727
  18. Transthyretin is expressed in the pineal gland >30-fold higher than the median expression level in other tissues, at levels similar to those in the choroid plexus. PMID: 19103603

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Transthyretin family
Tissue Specificity
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in the choroid plexus. Detected at lower levels in the liver.
Database Links
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1