Rat relaxin 1 (RLN1) ELISA kit

Code CSB-EL019749RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
relaxin 1
Alternative Names
Rln1 ELISA Kit; Rln ELISA Kit; Prorelaxin 1 [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
Abbreviation
RLN1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
2.4 pg/mL-150 pg/mL
Sensitivity
0.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-99  
1:2 Average % 89  
Range % 84-93  
1:4 Average % 92  
Range % 88-96  
1:8 Average % 97  
Range % 92-101  
Recovery
The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 105 100-110  
EDTA plasma (n=4) 84 80-88  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
150 2.643 2.487 2.565 2.439  
75 2.097 2.236 2.167 2.041  
37.5 1.468 1.551 1.510 1.384  
18.75 0.907 0.945 0.926 0.800  
9.4 0.583 0.617 0.600 0.474  
4.7 0.384 0.401 0.393 0.267  
2.4 0.235 0.252 0.244 0.118  
0 0.121 0.130 0.126    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat RLN1 ELISA Kit was designed for the quantitative measurement of Rat RLN1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 2.4 pg/mL-150 pg/mL and the sensitivity is 0.6 pg/mL.

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Target Background

Function
(From Uniprot)
Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
Gene References into Functions
  1. Relaxin-1 mRNA in the hearts of diabetic rats was significantly increased and that relaxin-3 mRNA expression at different time points was decreased. In addition, significant down-regulation of the mRNA expression of RXFP1 and RXFP3 was observed at 4 weeks after Streptozocin. PMID: 28776188
  2. The digestive tract has been shown to express relaxin receptors; the hormone appears to exert site-specific effects acting at the neural or at the smooth muscle level, mainly by a nitric oxide-mediated mechanism. (Review) PMID: 28606038
  3. We have shown that relaxin stimulates Sertoli cell proliferation through the activation of MEK/ERK1/2 and PI3K/AKT pathways. PMID: 24640565
  4. The aim of the present study was to explore a role of relaxin at specific stages of spermatogenesis using a co-culture of rat Sertoli and germ cells. PMID: 24640566
  5. Raxin is a mediator of the refractoriness of the mesangial cells to angiotensin II during pregnancy. PMID: 23207895
  6. Data demonstrate a functional role for relaxin in mediating uterine artery compliance in pregnant rats, which may be necessary to maintain adequate uterine blood flow to the uterus and placenta. PMID: 22744867
  7. A novel G-quadruplex in the relaxin gene, is reported. PMID: 22363579
  8. Rln-1 was localized in the granulosa cells of the ovarian follicles, and immunoreactivity against Rln 1 was increased by orthodontic tooth movement in a time-dependent manner. PMID: 21410550
  9. relaxin regulates fibroblast proliferation, differentiation, and collagen deposition and may have therapeutic potential in diseased states characterized by cardiac fibrosis. PMID: 15155573
  10. role for relaxin in the systemic hemodynamic changes of pregnancy, as well as novel therapeutic potential for relaxin in modifying arterial stiffness and cardiac afterload. PMID: 15198972
  11. High glucose concentration could stimulate the expression of endogenous relaxin. PMID: 19542742

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Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links
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