Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Instructions
Code CSB-E08134r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name platelet/endothelial cell adhesion molecule
Alternative Names Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
Abbreviation PECAM1
Uniprot No. Q3SWT0
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.468 2.554 2.511 2.372
100 1.656 1.732 1.694 1.555
50 1.033 1.067 1.050 0.911
25 0.591 0.611 0.601 0.462
12.5 0.380 0.398 0.389 0.250
6.25 0.278 0.286 0.282 0.143
3.12 0.203 0.207 0.205 0.066
0 0.138 0.139 0.139  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
Gene References into Functions
  1. Circulating PECAM-1 is a promising biomarker for the detection and monitoring of ventilator-induced lung injury. PMID: 24588994
  2. Shock wave therapy leads to mobilization of endogenous CD31/CD34-positive endothelial cells. PMID: 23395097
  3. PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in acute edematous pancreatitis PMID: 14599961
  4. A reverse expression of PECAM-1 on neutrophils was found between peripheral circulation and pancreatic microcirculation, so inhibition of PECAM-1 expression may improve the pathological change of acute edematous pancreatitis. PMID: 15655817
  5. expression pattern of PECAM & VE-cadherin at endothelial cell junctions in adult initial lymphatic network; results suggest that primary valves are associated with discontinuous expression of endothelial junction molecules PMID: 17935476

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Membrane raft, Cell junction
Database Links

KEGG: rno:29583

STRING: 10116.ENSRNOP00000044403

UniGene: Rn.1878

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