Rat α-glutathione S-transferases,α-GST ELISA Kit

Code CSB-E08907r
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Alternative Names
Gsta1 ELISA Kit; Glutathione S-transferase alpha-1 ELISA Kit; EC 2.5.1.18 ELISA Kit; 13-hydroperoxyoctadecadienoate peroxidase ELISA Kit; EC 1.11.1.- ELISA Kit; Androst-5-ene-3,17-dione isomerase ELISA Kit; EC 5.3.3.- ELISA Kit; GST 1-1 ELISA Kit; GST 1a-1a ELISA Kit; GST A1-1 ELISA Kit; GST B ELISA Kit; Glutathione S-transferase Ya-1 ELISA Kit; GST Ya1 ELISA Kit; Ligandin ELISA Kit
Abbreviation
Gsta1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 mU/mL-10 mU/mL
Sensitivity
0.039 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 84-96  
1:2 Average % 99  
Range % 92-105  
1:4 Average % 104  
Range % 100-107  
1:8 Average % 94  
Range % 89-99  
Recovery
The recovery of rat α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 82-89  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
10 2.588 2.599 2.594 2.394  
5 1.884 1.899 1.892 1.692  
2.5 1.265 1.287 1.276 1.076  
1.25 0.852 0.898 0.875 0.675  
0.625 0.588 0.598 0.593 0.393  
0.312 0.452 0.469 0.461 0.261  
0.156 0.374 0.397 0.386 0.186  
0 0.199 0.201 0.200    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat Gsta1 ELISA Kit was designed for the quantitative measurement of Rat Gsta1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL .

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Target Background

Function
(From Uniprot)
Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.
Gene References into Functions
  1. The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1. PMID: 21905055
  2. Mechanism of negative regulation of rat glutathione S-transferase A2 by the cytokine interleukin 6. PMID: 11939905
  3. Oltipraz-induced GSTA2 gene expression is dependent upon PI3-kinase-mediated nuclear translocation and binding of C/EBP-beta to the GSTA2 gene promoter. PMID: 12509401
  4. Single mutations are introduced to evaluate the importance of amino acids at the subunit interface of GSTA1-1. Phe52 and Arg69 are found to be major determinants of dimer formation: a single mutation at either position substantially hinders dimerization. PMID: 15035604
  5. P.893: Ceramide inhibits C/EBP-beta or Nrf2 activation, which contributes to repression of GSTA2 gene transactivation. P. 897: "...ceramide inhibition of... transcription factors contributes to the repression of the GSTA2 gene transactivation." PMID: 15319326
  6. C/EBP alpha and beta are involved in glucocorticoid-dependent repression of GSTA2 gene expression PMID: 17786629

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Subcellular Location
Cytoplasm.
Protein Families
GST superfamily, Alpha family
Database Links

KEGG: rno:24422

UniGene: Rn.144550

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