Sheep Immunoglobulin M(IgM) ELISA Kit

Instructions
Code CSB-E13682Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Immunoglobulin M(IgM)
Abbreviation IgM
Species Ovis aries (Sheep)
Sample Types serum, plasma
Detection Range 2.5 μg/mL-40 μg/mL
Sensitivity 1.25 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:20 Average % 87    
Range % 83-98    
1:40 Average % 95    
Range % 91-102    
1:80 Average % 95    
Range % 90-100    
1:100 Average % 90    
Range % 86-98    
Recovery
The recovery of sheep IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 87-105    
EDTA plasma (n=4) 95 90-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
μg/ml OD1 OD2 Average      
40 0.173 0.167 0.17      
20 0.268 0.275 0.272      
10 0.511 0.502 0.507      
5 0.979 0.942 0.961      
2.5 1.342 1.359 1.351      
0 2.285 2.299 2.292      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Is itpossible to perform this kit on bronchoalveolar lavage fluid?

A:
Thanks for your inquiry.
Here advice you to arrange preliminary experiment.
Here is our sample method for your reference:
Bronchoalveolar Lavage Fluid
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately or aliquot and store samples at -20°C.
Avoid repeated freeze-thaw cycles.
Pls let me know if you have any further questions. Thank you.

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  Email: [email protected]
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