Sheep adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-EQ027618SH
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
adrencocorticotropic hormone (ACTH)
Abbreviation
ACTH
Species
Ovis aries (Sheep)
Sample Types
serum, plasma
Detection Range
0.097 pg/mL-400 pg/mL
Sensitivity
0.097 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %82-89
1:2Average %91
Range %88-95
1:4Average %87
Range %83-95
1:8Average %100
Range %95-105
Recovery
The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9994-103
EDTA plasma (n=4)8987-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
4000.175 0.177 0.176
1000.254 0.268 0.261
250.335 0.353 0.344
6.250.582 0.598 0.590
1.560.913 0.934 0.924
0.391.461 1.498 1.480
0.0971.751 1.659 1.705
02.031 1.957 1.994
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Sheep ACTH ELISA Kit was designed for the quantitative measurement of Sheep ACTH protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.097 pg/mL-400 pg/mL and the sensitivity is 0.097 pg/mL.

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