Bovine adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E14322B
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation ACTH
Target Name adrenocorticotropic hormone (ACTH)
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 0.12 pg/mL-500 pg/mL
Sensitivity 0.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 83  
Range % 80-86  
1:2 Average % 84  
Range % 81-87  
1:4 Average % 95  
Range % 91-99  
1:8 Average % 92  
Range % 88-96  
Recovery
The recovery of bovine ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 93-99  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
500 0.200 0.204 0.202    
125 0.298 0.282 0.290    
31.25 0.440 0.465 0.453    
7.8 0.594 0.587 0.591    
1.95 0.806 0.852 0.829    
0.49 1.374 1.387 1.381    
0.12 1.503 1.526 1.515    
0 1.780 1.712 1.746    
References
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