Bovine adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E14322B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
adrenocorticotropic hormone (ACTH)
Abbreviation
ACTH
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
0.12 pg/mL-500 pg/mL
Sensitivity
0.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 83  
Range % 80-86  
1:2 Average % 84  
Range % 81-87  
1:4 Average % 95  
Range % 91-99  
1:8 Average % 92  
Range % 88-96  
Recovery
The recovery of bovine ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 93-99  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
500 0.200 0.204 0.202    
125 0.298 0.282 0.290    
31.25 0.440 0.465 0.453    
7.8 0.594 0.587 0.591    
1.95 0.806 0.852 0.829    
0.49 1.374 1.387 1.381    
0.12 1.503 1.526 1.515    
0 1.780 1.712 1.746    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine ACTH ELISA Kit was designed for the quantitative measurement of Bovine ACTH protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.12 pg/mL-500 pg/mL and the sensitivity is 0.12 pg/mL.

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