Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E06874m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name adrenocorticotropic hormone (ACTH)
Abbreviation ACTH
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %80-105
1:2Average %96
Range %85-104
1:4Average %98
Range %92-104
1:8Average %96
Range %82-110
The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9584-102
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
12003.134 3.126 3.130 2.988
6002.537 2.532 2.535 2.393
3001.754 1.765 1.760 1.618
1501.053 1.050 1.052 0.910
750.651 0.647 0.649 0.507
37.50.443 0.435 0.439 0.297
18.750.273 0.265 0.269 0.127
00.140 0.144 0.142  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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