Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

Home
Kit
ELISA Kits
Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Name: Mouse adrenocorticotropic hormone (ACTH) ELISA kit
Brand: CUSABIO
SKU: CSB-E06874m

Citations (6) Protocol MSDS

Code	CSB-E06874m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (6)
Related Products
Customer Reviews and Q&A

Target Name

adrenocorticotropic hormone (ACTH)

Abbreviation

ACTH

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.68 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	98
	Range %	92-104
1:8	Average %	96
	Range %	82-110

Recovery

The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	3.134	3.126	3.130	2.988
600	2.537	2.532	2.535	2.393
300	1.754	1.765	1.760	1.618
150	1.053	1.050	1.052	0.910
75	0.651	0.647	0.649	0.507
37.5	0.443	0.435	0.439	0.297
18.75	0.273	0.265	0.269	0.127
0	0.140	0.144	0.142

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The mouse ACTH ELISA kit (CSB-E06874m) is designed for the quantitative measurement of mouse ACTH protein in serum and plasma. It quantitates mouse ACTH with 4.68 pg/ml sensitivity and shows excellent specificity for mouse ACTH. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated ACTH antibody that recognizes the analyte bound by the immobilized ACTH antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of ACTH in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse ACTH in the sample.

ACTH is a tropic hormone synthesized in the basophilic cells of the anterior pituitary gland of the brain. It mainly functions to stimulate the production and release of cortisol from the cortex of the adrenal gland. ACTH is associated with Addison's disease, Cushing's syndrome, and Cushing's disease. It plays a role in glucose metabolism and immune function. Additionally, ACTH also participates in bone resorption, catabolism of proteins, anabolic effects on muscle and bones, as well as stimulation of spermatogenesis in men.

Excessive free fatty acid sensing in pituitary lactotrophs elicits steatotic liver disease by decreasing prolactin levels X Ji,Cell reports,2024
Single-cell transcriptomic analysis reveals rich pituitary–Immune interactions under systemic inflammation T Yan,PLoS biology,2023
Dendrobium officinale polysaccharides alleviate depression-like symptoms via regulating gut microbiota-neuroinflammation in perimenopausal mice QP Zhang,Journal of Functional Foods,2021
Intranasal Oxytocin Administration Ameliorates DSS-induced Abnormal Stress-related Behavior and Intestinal Inflammation in an IBD Mouse Model C Liu,Authorea Preprints,2020
Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice Cheng J.et al,Int Immunopharmacol.,2018
Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:4

Review: CSB-E06874m kit was used to detect the serum ACTH level of mice. After 4 times dilution, the serum ACTH level of the normal group, the model group and the administration group were mostly within the standard curve range, but if the sample was sufficient, 1:1 dilution or the use of stock solution could be considered, and the stock solution was still within the standard curve range after the conversion of dilution. All experiments were completed within 6 hours. The results were stable and the standard curves were good.

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.