The mouse ACTH ELISA kit (CSB-E06874m) is designed for the quantitative measurement of mouse ACTH protein in serum and plasma. It quantitates mouse ACTH with 4.68 pg/ml sensitivity and shows excellent specificity for mouse ACTH. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated ACTH antibody that recognizes the analyte bound by the immobilized ACTH antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of ACTH in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse ACTH in the sample.
ACTH is a tropic hormone synthesized in the basophilic cells of the anterior pituitary gland of the brain. It mainly functions to stimulate the production and release of cortisol from the cortex of the adrenal gland. ACTH is associated with Addison's disease, Cushing's syndrome, and Cushing's disease. It plays a role in glucose metabolism and immune function. Additionally, ACTH also participates in bone resorption, catabolism of proteins, anabolic effects on muscle and bones, as well as stimulation of spermatogenesis in men.