Mouse adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E06874m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
adrenocorticotropic hormone (ACTH)
Abbreviation
ACTH
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.68 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-105
1:2Average %96
Range %85-104
1:4Average %98
Range %92-104
1:8Average %96
Range %82-110
Recovery
The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9584-102
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12003.134 3.126 3.130 2.988
6002.537 2.532 2.535 2.393
3001.754 1.765 1.760 1.618
1501.053 1.050 1.052 0.910
750.651 0.647 0.649 0.507
37.50.443 0.435 0.439 0.297
18.750.273 0.265 0.269 0.127
00.140 0.144 0.142  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The mouse ACTH ELISA kit (CSB-E06874m) is designed for the quantitative measurement of mouse ACTH protein in serum and plasma. It quantitates mouse ACTH with 4.68 pg/ml sensitivity and shows excellent specificity for mouse ACTH. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated ACTH antibody that recognizes the analyte bound by the immobilized ACTH antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of ACTH in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse ACTH in the sample.

ACTH is a tropic hormone synthesized in the basophilic cells of the anterior pituitary gland of the brain. It mainly functions to stimulate the production and release of cortisol from the cortex of the adrenal gland. ACTH is associated with Addison's disease, Cushing's syndrome, and Cushing's disease. It plays a role in glucose metabolism and immune function. Additionally, ACTH also participates in bone resorption, catabolism of proteins, anabolic effects on muscle and bones, as well as stimulation of spermatogenesis in men.

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