Fish adrenocorticotropic hormone (ACTH) ELISA kit

Code CSB-E15926Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Fish ACTH ELISA Kit was designed for the quantitative measurement of Fish ACTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 75 pg/mL-1200 pg/mL and the sensitivity is 75 pg/mL.

Target Name adrenocorticotropic hormone (ACTH)
Abbreviation ACTH
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 75 pg/mL-1200 pg/mL
Sensitivity 75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 105  
Range % 94-109  
1:2 Average % 92  
Range % 84-97  
1:4 Average % 101  
Range % 98-105  
1:8 Average % 95  
Range % 84-98  
The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 82-97  
EDTA plasma (n=4) 94 90-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1200 0.149 0.152 0.151    
600 0.300 0.288 0.294    
300 0.477 0.491 0.484    
150 0.871 0.889 0.880    
75 1.835 1.887 1.861    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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