Human Parathyroid hormone,PTH ELISA Kit

Code CSB-E06934h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01270
Lead Time 3-5 working days
Abbreviation PTH
Target Name parathyroid hormone
Alias PTH1, parathormone|parathyrin|parathyroid hormone 1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a hormone secreted by parathyroid cells. This hormone elevates blood Ca2+ level by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH).
HGNC 9606
RGD 3440
MGI 97799
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-93  
1:2 Average % 104  
Range % 97-107  
1:4 Average % 95  
Range % 88-99  
1:8 Average % 100  
Range % 95-103  
Recovery
The recovery of human PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 110 104-113  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.674 2.598 2.636 2.523  
2000 2.165 2.134 2.150 2.037  
1000 1.348 1.333 1.341 1.228  
500 0.739 0.754 0.747 0.634  
250 0.347 0.334 0.341 0.228  
125 0.282 0.278 0.280 0.167  
62.5 0.189 0.183 0.186 0.073  
0 0.112 0.114 0.113    
References
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Function PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Involvement in disease Hypoparathyroidism, familial isolated (FIH)
Subcellular Location Secreted
Protein Families Parathyroid hormone family
Database Links

HGNC: 9606

OMIM: 146200

KEGG: hsa:5741

STRING: 9606.ENSP00000282091

UniGene: Hs.37045

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