Human transglutaminase 2C polypeptide,TGM2 ELISA Kit

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Target Data

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Product Details

Target Name

transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)

Alternative Names

Abbreviation

TGM2

Uniprot No.

P21980

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.68 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TGM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	86
	Range %	81-91
1:10	Average %	99
	Range %	93-102
1:20	Average %	99
	Range %	93-105
1:40	Average %	92
	Range %	86-95

Recovery

The recovery of human TGM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	97	93-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.736	2.654	2.695	2.563
600	2.413	2.321	2.367	2.235
300	1.908	1.854	1.881	1.749
150	0.967	0.988	0.978	0.846
75	0.698	0.702	0.700	0.568
37.5	0.388	0.413	0.401	0.269
18.75	0.277	0.264	0.271	0.139
0	0.134	0.129	0.132

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Data

Function

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

Protein Families

Transglutaminase superfamily, Transglutaminase family

Database Links

HGNC: 11778

OMIM: 190196

KEGG: hsa:7052

STRING: 9606.ENSP00000355330

UniGene: Hs.517033

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Check Code：

Code	CSB-E11797h
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.