Rabbit follicle-stimulating hormone,FSH ELISA Kit

Instructions
Code CSB-E06918Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name follicle-stimulating hormone,FSH
Alternative Names FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
Abbreviation FSH
Uniprot No. Q6IY73
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 mIU/mL-100 mIU/mL
Sensitivity 0.39 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-92
1:2Average %99
Range %91-105
1:4Average %103
Range %92-110
1:8Average %94
Range %86-98
Recovery
The recovery of rabbit FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9690-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
1002.885 2.878 2.882 2.789
502.346 2.221 2.284 2.191
251.619 1.603 1.611 1.518
12.50.951 0.933 0.942 0.849
6.250.468 0.451 0.460 0.367
3.120.255 0.243 0.249 0.156
1.560.159 0.149 0.154 0.061
00.096 0.090 0.093  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Subcellular Location Secreted.
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: ocu:100008946

STRING: 9986.ENSOCUP00000016404

UniGene: Ocu.3174

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