ABCB1 Monoclonal Antibody

Code CSB-MA001046A0m
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
    All lanes: ABCB1 antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 141 kDa
    Observed band size: 141-250 KDa
    Exposure time:5min
  • Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Full Product Name
Mouse anti-Homo sapiens (Human) ABCB1 Monoclonal antibody
Uniprot No.
Target Names
ABCB1
Alternative Names
ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
Raised in
Mouse
Species Reactivity
Human
Immunogen
Recombinant Human Calnexin protein (350-710AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG2b
Clone No.
5C12D3
Purification Method
>95%, Protein A purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF, FC
Recommended Dilution
Application Recommended Dilution
WB WB:1:1000-1:5000
IF 1:50-1:200
FC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The ABCB1 monoclonal antibody is created by immunizing mice with recombinant human ABCB1 protein (350-710aa) and then fusing the mouse B cells with mouse myeloma cells to generate hybridoma cells. ABCB1 antibody-secreting hybridomas are selected to culture in the mouse abdominal cavity. The ABCB1 monoclonal antibody is purified and isolated from the mouse ascites using protein A affinity chromatography to achieve a purity of over 95%. It is an unconjugated IgG2b antibody that can specifically recognize human ABCB1 protein and is well-suited for use in ELISA, WB, IF, and FC applications.

ABCB1 protein, also known as P-glycoprotein (P-gp), is a member of the ATP-binding cassette (ABC) transporter family that plays a critical role in the efflux of a wide range of endogenous and xenobiotic compounds out of cells. It is highly expressed in various tissues including the intestine, liver, and kidney, where it acts as a primary gatekeeper to prevent the entry of harmful substances into the body or promote their removal from the body.

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