Code | CSB-MA546610 |
Size | US$166 |
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Application | Recommended Dilution |
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IHC | 1:20-1:200 |
To generate the AMACR monoclonal antibody, mice are immunized with recombinant the synthesized peptide derived from human AMACR protein, and then B cells isolated from the immunized mice are fused with myeloma cells to create hybridomas. The hybridoma cells that produce the AMACR antibody are selected and cultured, and the AMACR monoclonal antibody is purified from the mouse ascites by affinity-chromatography using a specific immunogen. This unconjugated IgG1, Kappa antibody is suitable for detecting the human AMACR protein in ELISA and IHC assays.
AMACR is an enzyme involved in the beta-oxidation of branched-chain fatty acids and the metabolism of certain drugs and xenobiotics. It catalyzes the racemization of alpha-methyl branched-chain fatty acid coenzyme A (CoA) esters, converting the 2R-isomers to the 2S-omers. The expression of AMACR is increased in some cancers, particularly prostate cancer, and it is used as a diagnostic marker for this disease.
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