Code | CSB-MA555839 |
Size | US$166 |
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Application | Recommended Dilution |
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IHC | 1:20-1:200 |
To produce this KRT1 monoclonal antibody, B cells are isolated from the spleen of a mouse immunized with the synthesized peptide derived from human KRT1 protein and then fused with myeloma cells to create hybridoma cells. These hybridoma cells are screened to identify those that produce the KRT1 antibody. The selected hybridoma cells are then cultured in the mouse abdominal cavity, and the KRT1 monoclonal antibody is purified from the mouse ascites by affinity chromatography using a specific immunogen. The KRT1 monoclonal antibody is an unconjugated IgG1, Kappa antibody that is ideal for recognizing the human KRT1 protein in ELISA and IHC applications.
KRT1 is specifically expressed in the cornified layer of the epidermis and forms a network of intermediate filaments that provide mechanical strength and resilience to the skin. KRT1 is also involved in the regulation of cell migration, differentiation, and apoptosis. Mutations in the KRT1 gene have been linked to a variety of skin disorders, including epidermolytic hyperkeratosis and palmoplantar keratoderma.
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