MB Monoclonal Antibody

Datasheet
Code CSB-MA079101A0m
Product Type Monoclonal Antibody
Size US$350Purchase it in Cusabio online store
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Uniprot No. P02144
Image
  • Western Blot
    All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3µg/ml
    Lane 1: Mouse heart muscle lysate
    Lane 2: Recombinant Myoglobin at 10µg
    Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
    Predicted band size: 18 kDa
    Observed band size: 20 kDa
    Additional bands at: 120 kDa

  • Western Blot
    All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1µg/ml
    Lane 1: Myoglobin transfected 293 cell lysate
    Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
    Predicted band size: 18 kDa
    Observed band size: 20 kDa
    Additional bands at: 23, 25, 40 kDa

  • Immunohistochemical of paraffin-embedded human heart tissue using CSB-MA079101A0m at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-MA079101A0m at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Western Blot
    Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue
    All lanes: MB antibody at 1:2000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 18 kDa
    Observed band size: 18 kDa

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Myoglobin protein
Raised in Mouse
Species Reactivity Human, Mouse
Specificity Specific for Human Myoglobin denatured and native forms
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Relevance Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG1
Clonality Monoclonal
Alias Myoglobin; MB; MGC13548; PVALB
Immunogen Species Human
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 7A3D2
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Function Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Protein Families Globin family
Database Links

HGNC: 6915

OMIM: 160000

KEGG: hsa:4151

STRING: 9606.ENSP00000352835

UniGene: Hs.517586

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