Product Details

Uniprot No.

P02144

Target Names

Alternative Names

Myoglobin, MB

Species Reactivity

Human

Immunogen

A synthesized peptide from human MB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Myoglobin serves as the primary oxygen-carrying heme protein in cardiac and skeletal muscle tissue, playing an essential role in oxygen storage and facilitated diffusion within myocytes. As a well-established biomarker, myoglobin research spans cardiovascular disease investigation, muscle physiology studies, and clinical diagnostics development, making reliable detection tools indispensable for researchers working across these fields.

This recombinant rabbit monoclonal antibody, clone 12D2, offers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced through controlled expression systems, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The antibody is affinity-purified and supplied in a glycerol-containing buffer optimized for stability during storage.

Validation data demonstrates robust performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human tissues shows clear staining in both skeletal muscle and liver cancer samples at 1:100 dilution using citrate buffer antigen retrieval, confirming utility for tissue-based studies examining myoglobin expression patterns. Flow cytometry validation using the PC-3 prostate cancer cell line demonstrates effective intracellular detection with appropriate signal separation from isotype controls, supporting applications in cell-based expression analysis.

The antibody's validated compatibility with ELISA, immunohistochemistry, and flow cytometry provides workflow flexibility for researchers who need to examine myoglobin across different experimental contexts. Whether investigating muscle damage markers, characterizing tissue samples, or developing diagnostic assays, this recombinant monoclonal antibody delivers the consistency and multi-platform performance that demanding research applications require.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA283068A0HU
Size	US$210
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Image	IHC image of CSB-RA283068A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA283068A0HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing PC-3 cells stained with CSB-RA283068A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MB Recombinant Monoclonal Antibody

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