Human Myoglobin,MYO/MB ELISA Kit

Code CSB-E05141h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02144
Lead Time 3-5 working days
Protein Biological Process 2 Anion transport
Abbreviation MB
Protein Biological Process 1 Transport
Target Name myoglobin
Alias MGC13548, PVALB, OTTHUMP00000197922
Species Homo sapiens (Human)
Protein Biological Process 3 Oxygen transport
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.
HGNC 7969
RGD 621400
MGI 1346307
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:10Average %92
Range %89-99
1:20Average %87
Range %83-96
1:40Average %94
Range %90-100
1:80Average %94
Range %86-98
Recovery
The recovery of human MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9790-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.175 2.211 2.193 2.107
501.613 1.656 1.635 1.549
251.024 1.004 1.014 0.928
12.50.571 0.584 0.578 0.492
6.250.312 0.308 0.310 0.224
3.120.188 0.195 0.192 0.106
1.560.129 0.125 0.127 0.041
00.085 0.087 0.086
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Function Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Protein Families Globin family
Database Links

HGNC: 6915

OMIM: 160000

KEGG: hsa:4151

STRING: 9606.ENSP00000352835

UniGene: Hs.517586

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