PODXL Monoclonal Antibody

Datasheet
Code CSB-MA134471A0m
See More Details Free Antibody trial simple
Product Type Monoclonal Antibody
Size US$350
Uniprot No. O00592
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
    All lanes: PODXL antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size: 59, 56 kDa
    Observed band size: 59 kDa

  • Western Blot
    Positive WB detected in: A549 whole cell lysate
    All lanes: PODXL antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size: 59, 56 kDa
    Observed band size: 59 kDa

  • Western Blot
    Positive WB detected in: Mouse brain tissue
    All lanes: PODXL antibody at 0.43µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size: 59, 56 kDa
    Observed band size: 59 kDa

  • Western Blot
    Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
    All lanes: PODXL antibody at 1.28µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size: 59, 56 kDa
    Observed band size: 59 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

  • Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

  • Immunoprecipitating PODXL in HEK293 whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg)
    Lane 3: HEK293 whole cell lysate (10µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunogen Recombinant Human PODXL protein
Raised in Mouse
Species Reactivity Human, Mouse, Rat
Specificity Specific for PODXL denatured and native forms
Tested Applications ELISA, WB, IHC, IP, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200, IP:1:200-1:2000
Relevance Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG2b
Clonality Monoclonal
Alias Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1
Immunogen Species Human
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 4D2G7
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Function Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Subcellular Location Apical cell membrane, Cell projection, lamellipodium, Cell projection, filopodium, Cell projection, ruffle, Cell projection, microvillus, Membrane raft, Membrane, Single-pass type I membrane protein
Protein Families Podocalyxin family
Tissue Specificity Glomerular epithelium cell (podocyte).
Database Links

HGNC: 9171

OMIM: 602632

KEGG: hsa:5420

STRING: 9606.ENSP00000367817

UniGene: Hs.744213

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