Product Details

Description

The PODXL (podocalyxin-like) antibody is a monoclonal antibody identified as the IgG2b isotype. Its production begins with immunizing mice with the recombinant human PODXL protein, isolating the PODXL-producing B cells, then fusing the PODXL-producing B cells with myeloma cells, injecting the PODXL-producing hybridoma cells into the abdominal cavity of mice, and finally collecting mouse ascites. This PODXL antibody was purified from mouse ascites fluids by protein G. Its purification reaches up to 95%. It can react with samples containing the PODXL protein from humans, mice, and rats. The quality of this PODXL monoclonal antibody has been tested in ELISA, WB, IHC, IP, IF, and FC applications.

PODXL, a highly glycosylated type I transmembrane protein associated with CD34, is highly expressed in glomerular podocytes, vascular endothelium, hematopoietic cells, and breast epithelial cells. The high expression of PODXL in these tissues initiates the occurrence of many physiologic processes, such as hematopoiesis, leucocyte-endothelial cell interaction, as well as the regulation of vascular permeability and neural development. PODXL is also involved in the modulation of adhesion, cell morphology, and cancer progression.

Uniprot No.

O00592

Alternative Names

GCTM-2 antigen antibody; Gp2 antibody; Gp200 antibody; MGC138240 antibody; PC antibody; PCLP antibody; PCLP-1 antibody; PCLP1 antibody; Pcx antibody; Podocalyxin antibody; Podocalyxin like antibody; Podocalyxin like protein antibody; Podocalyxin-like protein 1 antibody; Podxl antibody; PODXL_HUMAN antibody

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human PODXL protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG2b

Clone No.

4D2G7

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function	Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Gene References into Functions	s-Podxl is independently associated with carotid IMT and might be used as a novel biomarker for cardiovascular disease. PMID: 29321582 Results show high podocalyxin expression in prostate cancer and can be used as a tumor marker for disease progression and patients stratification. PMID: 30396958 Summary of the structural features of podocalyxin glycoforms from embryonic and induced pluripotent stem cells (review). PMID: 28980094 This set of symptoms strikingly mimics previously reported mouse Podxl(-/-) embryos, emphasizing the essential function of PODXL in mammalian kidney development and highlighting this patient as a human PODXL-null model. PMID: 29244787 These results indicate that PcMab-47 is useful in detecting podocalyxin of oral cancers for immunohistochemical analysis PMID: 28873000 expression of PODXL associates with TAZ downstream gene expression. Suppression of PODXL induces phosphorylation of LATS1 and TAZ, and is accompanied with a decrease in TAZ protein expression. We speculate that changes in actin cytoskeleton may participate in PODXL-mediated TAZ signaling. PMID: 28946619 Results show that PODXL performs as a tumor promoter in gastric tumor cells (GC). PODXL is a target gene of miR-509-3-5P; the ectopic expression of miR-509-3-5P in GC cell lines inhibits the colony, motility and invasion abilities via negatively targeting PODXL. PMID: 28432273 Podocalyxin as a major pluripotent marker and novel keratan sulfate proteoglycan in human embryonic and induced pluripotent stem cells. PMID: 28078490 A novel frameshift mutation in PODXL seems to be the likely cause of ARJP in this family. PMID: 26864383 These findings suggest a potential functional link in colorectal cancer between PODXL, EGFR and BRAF. PMID: 27160084 PODXL enhances motility and invasiveness through an increase in gelsolin-actin interactions in cell protrusions. PMID: 27461278 Results identify an anti-metastatic miRNA, miR-5100, that decreases the metastatic ability of pancreatic cancer partially by suppressing expression of PODXL. PMID: 26892887 Results indicate that urinary podocalyxin is not only an early marker but also a treatment target for diabetic nephropathy (DN). PMID: 26635084 Obese subjects showed evidence of renal alteration through the detection of a higher number of urinary podocalyxin positive cells. PMID: 25905599 Data show that both mucin16 (MUC16) and podocalyxin (PODXL)-E-selectin-mediated interactions are mechanically stronger than like L-selectin interactions at the single-molecule level. PMID: 26329844 In gastric cancer, PODXL expression by the polyclonal antibody HPA2110 is an independent marker of poor prognosis. PMID: 26674770 PCX promotes cisplatin chemoresistance in osteosarcoma cells through a PI3Kdependent mechanism. PMID: 26017117 EZR, CLIC5 and PODXL are overexpressed in hepatocellular carcinoma and may have a role in cell migration and invasiveness PMID: 26135398 PODXL up-regulates the expression level of Bmi1 in OTSCC cells. PMID: 25915207 podocalyxin to be an independent factor for poor prognosis in PDAC. To our knowledge, this is the first such report of its prognostic value. PMID: 26053486 miR-125b was found to be important in arteriosclerosis obliterans by suppressing the expression of PODXL and may serve as a potential therapeutic target for the treatment of arteriosclerosis obliterans. PMID: 25738314 We show that podocalyxin plays a key role in the formation of primary tumors and distant tumor metastasis. PMID: 25887862 PCLP1 expressed on breast tumor cells may function as an immunomodulatory molecule, which may represent a mechanism to evade the immune response. PMID: 26276714 These findings show that overexpression of PODXL enhanced invadopodia formation and tumor metastasis by inducing Rac1/Cdc42/cortactin signaling network. PMID: 24970760 overexpression of PODXL may be associated with human oral squamous cell carcinoma aggressiveness PMID: 24821609 A variant form of PODXL remains the most likely candidate causing focal and segmental glomerulosclerosis in a family with autosomal dominant inheritance. PMID: 24048372 Suggest that analysis of PODXL expression in the primary tumour is sufficient for its use as a prognostic and treatment predictive biomarker in colorectal cancer, also in patients with metastatic disease. PMID: 23819542 Urinary Podocalyxin positive-element may be a noninvasive marker for the early stage of Diabetic nephropathy. PMID: 24075693 TRA-1-81 is expressed only in the cytoplasm of uterine smooth muscle neoplasms. PMID: 24143384 PODXL is expressed in glioblastoma multiforme stem-like cells and is involved in cell proliferation and oncosphere formation. PMID: 24146797 Membranous PODXL expression is an independent risk factor for progressive disease and death in patients with urothelial bladder cancer PMID: 23652315 The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. PMID: 23396057 PODXL protein expression was analyzed n tissue microarrays with colorectal cancer tumor samples. High PODXL protein expression was significantly associated with unfavourable clinicopathological characteristics in both cohorts. PMID: 22769594 PODXL has the potential to be a useful biomarker for identifying good prognosis patients in characteristically poor prognosis breast cancer groups and may impact treatment of women with this disease. PMID: 23288345 podocalyxin, a heavily glycosylated type 1 transmembrane protein, is a glycoprotein ligand of recombinant N-terminal domain of the lectin BC2L-C from Burkholderia cenocepacia on human induced pluripotent stem cells and embryonic stem cells. PMID: 23526252 Levels of urinary PCX and the number of urinary podocytes are associated with histologic abnormalities in adults with IgA nephropathy. PMID: 22700887 Sialofucosylated PODXL is a functional E-and L-selectin ligand expressed by metastatic pancreatic cancer cells. PMID: 22814396 Most AML patients had a significant decrease in miR-199b-5p levels with elevated PODXL & DDR1 expressions. Both PODXL & DDR1 are targets of miR-199b-5p. PMID: 22374871 podocalyxin's ability to facilitate the formation of non-adhesive membrane domains may contribute to the formation of free-floating high grade serous tumor nodules during the initial steps of transperitoneal metastasis. PMID: 22262060 Podocalyxin-like 1 expression is an independent factor of poor prognosis in colorectal carcinoma. PMID: 21829192 The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, alpha-actin4, and podocin were found to increase with the progression of diabetic nephropathy. PMID: 21655212 This report identifies DNA methylation, miR-199a dysregulation and PODXL as critical factors in tumor malignancy. PMID: 21383689 PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression. PMID: 21390327 intact PODXL is released to the extracellular space as a cargo of microvesicles and also as a soluble cleaved fragment of ectodomain PMID: 21616097 Data show that podocalyxin overexpression in human embryonic kidney cells up-regulates Rac1 activity, which depends on a complex formed by podocalyxin, ERM-binding phosphoprotein 50, ezrin, and ARHGEF7, a Rac1 activator. PMID: 20395446 In pluripotent stem cells and in human cancer disease, podocalyxin may function in part to regulate and maintain the cell surface expression of the glucose-3-transporter. PMID: 20599725 podocalyxin has a role in defining the structure of the podocyte basal surface PMID: 15226400 Overexpression of the anti-adhesin podocalyxin is associated with breast cancer progression PMID: 15289306 PODXL variants are implicated in prostate neoplasm aggressiveness, with gene mapping to chromosome 7q32-q33. PMID: 16434482 Transcriptional regulation of Podxl is supported primarily by Sp1 site(s) and that DNA-methylation of the CpG promoter islands contributes to control the tissue specific expression of podxl. PMID: 16684343 Show More Hide All
Subcellular Location	Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane; Single-pass type I membrane protein.
Protein Families	Podocalyxin family
Tissue Specificity	Glomerular epithelium cell (podocyte).
Database Links	HGNC: 9171 OMIM: 602632 KEGG: hsa:5420 STRING: 9606.ENSP00000367817 UniGene: Hs.744213

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Code	CSB-MA134471A0m
Size	US$350
Image	Western Blot Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate All lanes: PODXL antibody at 2.5µg/ml Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size: 59, 56 kDa Observed band size: 59 kDa Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PODXL antibody at 2.5µg/ml Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size: 59, 56 kDa Observed band size: 59 kDa Western Blot Positive WB detected in: Mouse brain tissue All lanes: PODXL antibody at 0.43µg/ml Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size: 59, 56 kDa Observed band size: 59 kDa Western Blot Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue All lanes: PODXL antibody at 1.28µg/ml Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size: 59, 56 kDa Observed band size: 59 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200 Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200 Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating PODXL in HEK293 whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
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PODXL Monoclonal Antibody

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