lacZ Monoclonal Antibody

Datasheet

Code	CSB-MA009476A0m
Size	US$210
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 117 kDa Observed band size: 130 kDa Western Blot Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng) All lanes: lacZ antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 117 kDa Observed band size: 130 kDa Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody Immunoprecipitating lacZ in 293T transfected whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg) Lane 3: 293T transfected whole cell lysate (20µg) For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
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Target Background

Product Details

Full Product Name

Mouse anti-Escherichia coli lacZ Monoclonal antibody

Uniprot No.

P00722

Target Names

lacZ

Alternative Names

lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC 3.2.1.23 antibody; Lactase antibody

Raised in

Mouse

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

3B7D5

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, IP, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:64000
IF	1:50-1:200
IP	1ul-4ul
FC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production process of this lacZ monoclonal antibody involves immunizing mice with the recombinant Escherichia coli Beta-galactosidase protein (2-1024aa), followed by isolation splenocytes from immunized mice and fusion of splenocytes with myeloma cells to form hybridomas. The selected lacZ antibody-secreting hybridomas were cultured to harvest large amounts of lacZ monoclonal antibodies. This lacZ monoclonal antibody was purified with protein G with a purity of above 95%. It can be used for the detection of Escherichia coli lacZ protein in ELISA, WB, IF, IP, and FC applications.

lacZ protein is a β-galactosidase enzyme that hydrolyzes the glycosidic bond between a galactose and a glucose or galactose. Its main function is to break down lactose into glucose and galactose, which can then be utilized by the cell for energy production. Additionally, the lacZ protein is often used as a reporter gene in molecular biology experiments to detect the expression of genes of interest.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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