lacZ Monoclonal Antibody

Datasheet
Code CSB-MA009476A0m
Product Type Monoclonal Antibody
Size
Uniprot No. P00722
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa

  • Western Blot
    Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
    All lanes: lacZ antibody at 1:2000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa

  • Immunofluorescence staining of 293 transfected cells with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating lacZ in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA009476A0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

  • Overlay histogram showing 293 transfected cells stained with CSB-MA009476A0m (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunogen Recombinant Escherichia coli Beta-galactosidase protein
Raised in Mouse
Species Reactivity Escherichia coli
Tested Applications ELISA, WB, IF, IP, FC; Recommended dilution: WB:1:1000-1:64000, IF:1:50-1:200, IP:1:200-1:2000
Relevance Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG1
Clonality Monoclonal
Alias Beta-galactosidase (Beta-gal) (Lactase), lacZ
Immunogen Species Escherichia coli
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow Cytometry (FC) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 3B7D5
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