lacZ Monoclonal Antibody

Code CSB-MA009476A0m
Size US$210
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa
  • Western Blot
    Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
    All lanes: lacZ antibody at 1:2000
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa
  • Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
  • Immunoprecipitating lacZ in 293T transfected whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
    Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)
    Lane 3: 293T transfected whole cell lysate (20µg)
    For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
  • Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
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Product Details

Full Product Name
Mouse anti-Escherichia coli lacZ Monoclonal antibody
Uniprot No.
Target Names
Alternative Names
lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC antibody; Lactase antibody
Raised in
Species Reactivity
Escherichia coli
Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)
Immunogen Species
Escherichia coli
Clone No.
Purification Method
>95%, Protein G purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Tested Applications
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:64000
IF 1:50-1:200
IP 1ul-4ul
FC 1:100-1:300
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The production process of this lacZ monoclonal antibody involves immunizing mice with the recombinant Escherichia coli Beta-galactosidase protein (2-1024aa), followed by isolation splenocytes from immunized mice and fusion of splenocytes with myeloma cells to form hybridomas. The selected lacZ antibody-secreting hybridomas were cultured to harvest large amounts of lacZ monoclonal antibodies. This lacZ monoclonal antibody was purified with protein G with a purity of above 95%. It can be used for the detection of Escherichia coli lacZ protein in ELISA, WB, IF, IP, and FC applications.

lacZ protein is a β-galactosidase enzyme that hydrolyzes the glycosidic bond between a galactose and a glucose or galactose. Its main function is to break down lactose into glucose and galactose, which can then be utilized by the cell for energy production. Additionally, the lacZ protein is often used as a reporter gene in molecular biology experiments to detect the expression of genes of interest.

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