lacZ Monoclonal Antibody

Datasheet
Code CSB-MA009476A0m
Size US$350
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa

  • Western Blot
    Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
    All lanes: lacZ antibody at 1:2000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 117 kDa
    Observed band size: 130 kDa

  • Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody

  • Immunoprecipitating lacZ in 293T transfected whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
    Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)
    Lane 3: 293T transfected whole cell lysate (20µg)
    For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
     

  • Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.

Product Details

Uniprot No. P00722
Alternative Names Beta-galactosidase (Beta-gal) (Lactase), lacZ
Raised in Mouse
Species Reactivity Escherichia coli
Immunogen Recombinant Escherichia coli Beta-galactosidase protein
Immunogen Species Escherichia coli
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 3B7D5
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP, FC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:64000
IF 1:50-1:200
IP 1ul-4ul
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.