Proteinase K Recombinant Protein

SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
According to the left SDS-PAGE image, the purity of Proteinase K is 95%+ and the molecular weight is 28.9 kDa.

Detect Nucleic acid residue by agarose gel electrophores

Detect DNase residue by agarose gel electrophores

Detect RNase residue by agarose gel electrophores

Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated ＞30U/mg. Unit definition:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C

The Enzyme Activity Stability of Proteinase K

The Proteinase K powder was stored at 4℃. And its enzyme activity was assayed every month over the past year.
The left chart shows how its enzyme activity changes in a year. It turns out the enzyme activity is maintained more than 95%, which indicates that proteinase K is very stable.

The Proteinase K activity comparison of 3 vendors and CUSABIO

CUSABIO performed a Proteinase K activity comparison test. As the left chart shows, the activity of CUSABIO Proteinase K is better than that of the 3 vendors under the same testing conditions.