Proteinase K Recombinant Protein

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Code CSB-AP361972TIQ
Size $45
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Image
  • SDS-PAGE

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    According to the left SDS-PAGE image, the purity of Proteinase K is 95%+ and the molecular weight is 28.9 kDa.

  • Detect Nucleic acid residue by agarose gel electrophores

  • Detect DNase residue by agarose gel electrophores

  • Detect RNase residue by agarose gel electrophores

  • Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg. Unit definition:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C

  • The Enzyme Activity Stability of Proteinase K

    The Proteinase K powder was stored at 4℃. And its enzyme activity was assayed every month over the past year.
    The left chart shows how its enzyme activity changes in a year. It turns out the enzyme activity is maintained more than 95%, which indicates that proteinase K is very stable.

  • The Proteinase K activity comparison of 3 vendors and CUSABIO

    CUSABIO performed a Proteinase K activity comparison test. As the left chart shows, the activity of CUSABIO Proteinase K is better than that of the 3 vendors under the same testing conditions.

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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg.
Target Names
PROK
Uniprot No.
Alternative Names
Endopeptidase K,Tritirachium alkaline proteinase
Species
Tritirachium album (Engyodontium album)
Mol. Weight
28.9 kDa
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Applications : Antigen, Drug related studies, Protein structure analysis (Crystal / electron microscope) 

Review: Used proteinase K to improve the purity of the extracted DNA from a Bacillus thermoamylovorus strain producing a thermotolerant lipase by digesting contaminating cell proteins. To check the effectiveness of proteinase K to inhibit the activity of purified lipase in the absence and presence of PMSF.

By Shamsher S Kanwar

Target Background

Function
Hydrolyzes keratin at aromatic and hydrophobic residues.
Protein Families
Peptidase S8 family
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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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