Recombinant Finegoldia magna ATCC 53516 LPXTG-motif cell wall anchor domain protein, partial (Active)

Finegoldia magna ATCC 53516 LPXTG-motif cell wall anchor domain protein is a critical bacterial surface protein responsible for covalently anchoring effector proteins to the peptidoglycan cell wall, playing essential roles in bacterial adhesion, tissue colonization and host-pathogen interactions, making it a valuable research target for investigating gram-positive bacterial pathogenesis and developing novel antibacterial strategies. This mammalian-expressed partial construct spanning residues 106–470 carries an N-terminal 10×His tag for efficient purification, preserving the native folding required for authentic ligand and antibody recognition. Functional ELISA validation demonstrates specific binding to an anti-protein L recombinant antibody (CSB-RA006163MA1HU) with an EC50 of 1.601–1.944 ng/mL when immobilized at 2 μg/mL, providing quantitative evidence that this active protein supports antibody epitope mapping, competitive inhibition screening, and blocking antibody validation assays. The combination of >95% purity verified independently by both SDS-PAGE and SEC-HPLC meets the rigorous quality thresholds commonly required for surface plasmon resonance affinity characterization, biolayer interferometry kinetic studies, and use as a positive control in bacterial cell wall protein binding and functional assays.