Recombinant Finegoldia magna ATCC 53516 LPXTG-motif cell wall anchor domain protein, partial (Active)

Protein L, an LPXTG-motif cell wall anchor domain protein from Finegoldia magna ATCC 53516, specifically recognizes and binds to the kappa light chain variable region of immunoglobulins across multiple species and isotypes without interacting with the Fc fragment, making it a critical tool in antibody research, purification and diagnostic development. This E. coli-expressed partial protein spanning residues 106–470 carries a C-terminal 6×His tag for facile purification and has an apparent molecular weight of approximately 47.4 kDa. Functional ELISA validation confirms that immobilized Protein L at a concentration of 2 μg/mL exhibits specific binding to an anti-CTLA4 recombinant antibody (CSB-RA006163MA2HU) with an EC50 value ranging from 1.764 to 2.413 ng/mL, demonstrating that the protein retains the native conformational structure essential for high-affinity antibody interaction. With a purity exceeding 85% as determined by SDS-PAGE and provided as a stable lyophilized powder, this active recombinant Protein L is well-suited for various applications including antibody affinity purification, immunoprecipitation, Western blotting, therapeutic antibody screening, and kinetic binding characterization via surface plasmon resonance or biolayer interferometry.