Recombinant Finegoldia magna ATCC 53516 LPXTG-motif cell wall anchor domain protein, partial,Biotinylated (Active)

The LPXTG-motif cell wall anchor domain from Finegoldia magna ATCC 53516 exhibits robust immunoglobulin-binding capacity, a property that positions this protein as a valuable tool for antibody capture and detection workflows. Functional ELISA validation demonstrates specific binding to immobilized anti-CTLA4 antibody with an EC50 of 1.123–1.761 ng/mL, confirming its utility as a detection reagent in binding assays and as a positive control in immunoassay development. Expressed in E. coli as a biotinylated construct spanning residues 106–470 with N-terminal 10xHis-Avi tags, this protein achieves greater than 95% purity by SDS-PAGE and maintains endotoxin levels below 1.0 EU/μg, meeting the quality thresholds commonly required for antibody characterization studies and assay optimization experiments. The site-specific biotinylation enables straightforward immobilization on streptavidin surfaces, supporting applications in surface plasmon resonance, ELISA-based antibody screening, and multiplex immunoassay controls where reproducible orientation and presentation are critical.