Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2 μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU), the EC50 is 14.09-23.17 ng/mL.

Target Names

ALPG

Uniprot No.

P10696

Alternative Names

(ALP-1)(Alkaline phosphatase Nagao isozyme)(Alkaline phosphatase, placental-like)(Germ cell alkaline phosphatase )(Placental alkaline phosphatase-like)

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

20-503aa

Target Protein Sequence

IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD

Mol. Weight

53.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human ALPG recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human ALPG (20-503aa) was expressed with the C-terminal 10xHis tag. The purity of this ALPG protein was greater than 95%. And the activity was validated. Its endotoxin content is less than 1.0 EU/ug as determined by the LAL method. It has be validated as an active protein. In a functional ELISA, immobilized human ALPG at 2 μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU) with the EC₅₀ of 14.09-23.17 ng/mL. It would be a very helpful tool for your research.

Alkaline Phosphatase (AP/ALP/AKP/ALKP/ALPase/Alk Phos) is often called EC 3.1.3.1. At present, six isozymes of AKP1, AKP2, AKP3, AKP4, AKP5 and AKP6 (or ALP1, ALP2, ALP3, ALP4, ALP5 and ALP6) have been found. Among them, the 1st, 2nd, and 6th types are all from the liver, the 3rd type is from bone cells, the 4th type is produced from the placenta and cancer cells, and the 5th type is from the small intestinal villous epithelium and fibroblasts. ALPG is a highly specific tumor cell surface antigen, and its unique expression pattern is very suitable for the development of therapeutic drugs against tumors expressing ALPPL2, and is a potential therapeutic target. The development of active lactational ALPG protein can provide preliminary support for in-depth study of the function of ALPG and the development of tumor drugs.

Target Background

Function

Alkaline phosphatase that can hydrolyze various phosphate compounds.

Gene References into Functions

aptamer-mediated identification of ALPPL-2 on the cell surface and cell secretions of pancreatic cancer cells supports its potential use in the serum- and membrane-based diagnosis of PDAC PMID: 23467613
These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. PMID: 16412386
Premature induction of hypertrophy-related molecules (type X collagen and matrix metalloproteinase 13) occurred before production of type II collagen and was followed by up-regulation of alkaline phosphatase activity. PMID: 17009260

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Alkaline phosphatase family

Tissue Specificity

Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Database Links

HGNC: 441

OMIM: 171810

KEGG: hsa:251

STRING: 9606.ENSP00000295453

UniGene: Hs.333509

Code	CSB-MP001633HU
Abbreviation	Recombinant Human ALPG protein (Active)
MSDS	MSDS Datasheet
Size	$136
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU)， the EC₅₀ is 14.09-23.17 ng/mL. Biological Activity Assay
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Recombinant Human Alkaline phosphatase, germ cell type (ALPG) (Active)

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