Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2 μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU), the EC50 is 14.09-23.17 ng/mL.
Alternative Names
(ALP-1)(Alkaline phosphatase Nagao isozyme)(Alkaline phosphatase, placental-like)(Germ cell alkaline phosphatase )(Placental alkaline phosphatase-like)
Species
Homo sapiens (Human)
Expression Region
20-503aa
Target Protein Sequence
IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Human ALPG recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human ALPG (20-503aa) was expressed with the C-terminal 10xHis tag. The purity of this ALPG protein was greater than 95%. And the activity was validated. Its endotoxin content is less than 1.0 EU/ug as determined by the LAL method. It has be validated as an active protein. In a functional ELISA, immobilized human ALPG at 2 μg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU) with the EC50 of 14.09-23.17 ng/mL. It would be a very helpful tool for your research.
Alkaline Phosphatase (AP/ALP/AKP/ALKP/ALPase/Alk Phos) is often called EC 3.1.3.1. At present, six isozymes of AKP1, AKP2, AKP3, AKP4, AKP5 and AKP6 (or ALP1, ALP2, ALP3, ALP4, ALP5 and ALP6) have been found. Among them, the 1st, 2nd, and 6th types are all from the liver, the 3rd type is from bone cells, the 4th type is produced from the placenta and cancer cells, and the 5th type is from the small intestinal villous epithelium and fibroblasts. ALPG is a highly specific tumor cell surface antigen, and its unique expression pattern is very suitable for the development of therapeutic drugs against tumors expressing ALPPL2, and is a potential therapeutic target. The development of active lactational ALPG protein can provide preliminary support for in-depth study of the function of ALPG and the development of tumor drugs.
