Recombinant Human Alkaline phosphatase, germ cell type (ALPG) (Active)

ALPG, a germ cell-specific alkaline phosphatase isoenzyme overexpressed in certain malignancies, serves as both a cancer biomarker and a functional enzyme target in oncology research. This full-length mature protein (aa 20–503) with C-terminal 10xHis tag demonstrates quantifiable binding activity in functional ELISA, achieving an EC50 of 14.09–23.17 ng/mL with anti-ALPG recombinant antibody, which supports its use as a positive control in enzyme-linked immunoassays and validates its structural integrity for antibody-antigen interaction studies. The protein meets the purity and endotoxin criteria typical for inhibitor screening and IC50 determination experiments, with >95% purity by SDS-PAGE and <1.0 EU/μg endotoxin, providing a suitable basis for drug candidate evaluation where low contaminant levels are essential to avoid interference in dose-response measurements. Researchers investigating ALPG's role in germ cell tumors or placental-like alkaline phosphatase expression in cancer can apply this reagent to substrate specificity profiling and kinetic parameter analysis once enzymatic activity against phosphate substrates is characterized in their assay conditions.