Recombinant Human Carbonic anhydrase 7 (CA7) (Active)

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Code CSB-AP005411HU
MSDS
Size $116
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
The esterase activity is determined to be greater than 1000 pmol/min/ug
Target Names
CA7
Uniprot No.
Research Area
Cell Biology
Alternative Names
CA 7; CA VII; CA-VII; Ca7; CAH7_HUMAN; Carbonate dehydratase VII; Carbonic anhydrase 7; Carbonic anhydrase VII; Carbonic dehydratase VII; CAVII; EC=4.2.1.1
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-264aa
Complete Sequence
MTGHHGWGYGQDDGPSHWHKLYPIAQGDRQSPINIISSQAVYSPSLQPLELSYEACMSLSITNNGHSVQVDFNDSDDRTVVTGGPLEGPYRLKQFHFHWGKKHDVGSEHTVDGKSFPSELHLVHWNAKKYSTFGEAASAPDGLAVVGVFLETGDEHPSMNRLTDALYMVRFKGTKAQFSCFNPKCLLPASRHYWTYPGSLTTPPLSESVTWIVLREPICISERQMGKFRSLLFTSEDDERIHMVNNFRPPQPLKGRVVKASFRA
Mol. Weight
30.72 kDa
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid
Buffer
0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Reversible hydration of carbon dioxide.
Gene References into Functions
  1. Our results indicate that decreased expression of CA7 correlates with disease progression and predicts poor prognosis in CRC, especially for patients with early stage tumors. PMID: 25885898
  2. native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process PMID: 23851572
  3. conclude that the full-length CA VII is a predominant active form in human brain and also in other tissues. In addition to the brain, CA VII is expressed in several other organs including the stomach, duodenum, colon, liver, and skeletal muscle PMID: 20493921
  4. GABAergic transmission is influenced by the neuronal expression of carbonic anhydrase cavii--REVIEW PMID: 15528236
  5. these data furnish further evidence that hCA VII is the isozyme responsible for the anticonvulsant/antiepileptic activity of sulfonamides and sulfamates PMID: 15686895

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Subcellular Location
Cytoplasm.
Protein Families
Alpha-carbonic anhydrase family
Database Links

HGNC: 1381

OMIM: 114770

KEGG: hsa:766

STRING: 9606.ENSP00000345659

UniGene: Hs.37014

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