Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 μg/ml.
Research Area
Stem Cells?
Alternative Names
C78960; Desert hedgehog (Drosophila) homolog; Desert hedgehog; Desert hedgehog homolog (Drosophila); Desert hedgehog protein C-product; Desert hedgehog protein precursor; DHH; DHH_HUMAN; GDXYM; HHG-3; Hira; SRXY7
Species
Homo sapiens (Human)
Expression Region
24-198aa
Complete Sequence
II+GPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG
Buffer
Lyophilized from a 0.2 μm filtered 10mM PB, pH 6.0, 300mM NaCl
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Dive into stem cell research with our premium Recombinant Human DHH protein, also known as Desert hedgehog protein or HHG-3. Sourced from E.coli, this partial protein (24-198aa) is provided in a tag-free format, ensuring a native-like structure and function. With a purity of >96% as confirmed by SDS-PAGE and HPLC, and endotoxin levels <1.0 EU/µg as determined by the LAL method, this protein guarantees minimal interference in your experiments. Recombinant Human DHH is fully biologically active, as demonstrated by its ability to induce alkaline phosphatase production by C3H10T1/2 (CCL-226) cells with an ED50 of 15-45 µg/ml. Delivered as lyophilized powder, our Recombinant Human DHH protein empowers you to advance your stem cell research with consistent results and the precision scientific quality you can trust.