The recombinant human IGFLR1 protein production follows a workflow of gene cloning, plasmid construction, protein expression, purification, and analysis. It is expressed in mammalian cells. Its expression region corresponds to the 23-163aa of the human IGFLR1. It is fused with a hFc-tag at the C-terminus. The resulting recombinant IGFLR1 protein is purified using affinity chromatography. SDS-PAGE analysis confirms the IGFLR1 protein purity exceeds 95%, and the LAL method detects its endotoxin levels of <1.0 EU/μg. Functional ELISA shows its specific binding with the human IGFL1 (CSB-MP764932HUh8), with an EC50 of 4.640-5.722 ng/mL.
IGFLR1 is a transmembrane protein primarily expressed on T cells [1]. It bears structural similarities to the TNFR family members, and human and mouse IGFLR1 share 61% amino acid sequence identity [1].
IGFLR1 is most abundantly expressed on T cells in mice, and in both human and mouse skin models, its expression is induced under inflammatory conditions, suggesting it may act as a regulatory element in T cell response in the skin [2]. Several studies have found that IGFLR1 is associated with immune regulation and infiltration in various cancers, including clear cell renal cell carcinoma, colorectal cancer, and non-small cell lung cancer [3][4][5][6]. IGFLR1 expression is elevated on the surface of CD8+ T cells in colorectal cancer, where it may contribute to T cell exhaustion [3]. Additionally, a specific cluster of TH1-like T cells co-expressing CXCL13 and BHLHE40 and exhibiting high levels of IGFLR1 was linked to microsatellite-instable colorectal tumors [4][5][6].
References:
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[2] R. Ehmann, K. Brandes, M. Antwerpen, M. Walter, K. Schlippenbach, E. Stegmaier, et al. Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus, Journal of General Virology, vol. 102, no. 3, 2021. https://doi.org/10.1099/jgv.0.001357
[3] W. Song, Y. Shao, X. He, P. Gong, Y. Yang, S. Huang, et al. Igflr1 as a novel prognostic biomarker in clear cell renal cell cancer correlating with immune infiltrates, Frontiers in Molecular Biosciences, vol. 7, 2020. https://doi.org/10.3389/fmolb.2020.565173
[4] N. Wang, R. Wang, X. Li, Z. Song, L. Xia, J. Wang, et al. Tumor microenvironment profiles reveal distinct therapy-oriented proteogenomic characteristics in colorectal cancer, Frontiers in Bioengineering and Biotechnology, vol. 9, 2021. https://doi.org/10.3389/fbioe.2021.757378
[5] L. Zhang and Z. Zhang. Recharacterizing tumor-infiltrating lymphocytes by single-cell rna sequencing, Cancer Immunology Research, vol. 7, no. 7, p. 1040-1046, 2019. https://doi.org/10.1158/2326-6066.cir-18-0658
[6] Y. Li, J. Jin, & F. Bai. Cancer biology deciphered by single-cell transcriptomic sequencing, Protein & Cell, vol. 13, no. 3, p. 167-179, 2021. https://doi.org/10.1007/s13238-021-00868-1
