Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein(MANF) (Active)

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Code CSB-AP002891HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of >50 IU/mg.
Target Names MANF
Uniprot No. P55145
Research Area Neuroscience
Alternative Names Arginine rich mutated in early stage tumors; Arginine rich protein; Arginine-rich protein; ARMET; ARMET protein; ARP; Manf; MANF_HUMAN; Mesencephalic astrocyte derived neurotrophic factor; Mesencephalic astrocyte-derived neurotrophic factor; MGC142148; MGC142150; Protein ARMET
Species Homo sapiens (Human)
Source E.coli
Expression Region 25-182aa
Mol. Weight 18.2 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

(From Uniprot)
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.
Gene References into Functions
  1. Our data indicate that increased MANF concentrations in serum are associated with the clinical manifestation of type 1 diabetes in children, but the exact mechanism behind the increase remains elusive PMID: 27356471
  2. Sulfatide binding promotes cellular MANF uptake and cytoprotection from hypoxia-induced cell death. PMID: 29497057
  3. Serum MANF level was higher in patients with newly diagnosed prediabetes and T2 Diabetes Mellitus than in normal glucose tolerance controls. MANF appears to be associated with Matsuda Index, QUICKI and HOMA-IR in prediabetes patients. PMID: 28216543
  4. the role of two short sequence motifs within the carboxy-(C) terminal domain of MANF in its neuroprotective activity, was studied. PMID: 26720341
  5. the selective expression of MANF in splenocytes may be involved in plasma cell differentiation and immune regulation. PMID: 26429332
  6. MANF is a protein that interacts with RTN1-C PMID: 25543119
  7. We demonstrate that Armet and Creld2 are genotype-specific ER stress response proteins with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in MED that could be exploited as a potential therapeutic target PMID: 23956175
  8. MANF binding to the plasma membrane also required the RTDL sequence and was inhibited with a peptide known to interact with KDELRs, suggesting MANF binds KDELRs at the surface. PMID: 23255601
  9. MANF and C-MANF protect neurons intracellularly as efficiently as Ku70. PMID: 21047780
  10. Pretreatment with adeno-associated-virus vector containing human MANF reduces the volume of cerebral infarction and facilitates behavioral recovery in experimental stroke rats. PMID: 20685313
  11. Armet is a novel secreted mediator of the adaptive pathway of unfolded protein response. PMID: 18561914
  12. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. PMID: 18718866
  13. structures of MANF and CDNF were solved; structure explains why MANF and CDNF are bifunctional; neurotrophic activity may reside in the N-terminal domain and ER stress response in the C-terminal domain PMID: 19258449

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Subcellular Location Secreted
Protein Families ARMET family
Database Links

HGNC: 15461

OMIM: 601916

KEGG: hsa:7873

STRING: 9606.ENSP00000432799

UniGene: Hs.436446


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