Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human MUC13 at 2 μg/mL can bind Anti-MUC13 recombinant antibody (CSB-RA887973MA1HU). The EC50 is 2.592-2.923 ng/mL.

Target Names

MUC13

Uniprot No.

Q9H3R2

Alternative Names

DRCC1, RECC

Species

Homo sapiens (Human)

Source

yeast

Expression Region

19-421aa

Target Protein Sequence

TNQGNSADAVTTTETATSGPTVAAADTTETNFPETASTTANTPSFPTATSPAPPIISTHSSSTIPTPAPPIISTHSSSTIPIPTAADSESTTNVNSLATSDIITASSPNDGLITMVPSETQSNNEMSPTTEDNQSSGPPTGTALLETSTLNSTGPSNPCQDDPCADNSLCVKLHNTSFCLCLEGYYYNSSTCKKGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDKFVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQRPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL

Mol. Weight

44.6 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

MUC13 is a transmembrane mucin overexpressed in epithelial cancers, where it modulates cell signaling and contributes to tumor progression, making validated recombinant forms valuable for investigating its role in oncogenic pathways. This yeast-expressed fragment spanning residues 19–421 demonstrates robust antibody-binding activity in functional ELISA, achieving an EC50 of 2.592–2.923 ng/mL when immobilized at 2 μg/mL and challenged with anti-MUC13 recombinant antibody, confirming proper folding and epitope accessibility. The quantified binding kinetics support its use in antibody development workflows, including screening hybridoma clones or validating therapeutic candidates, as well as in binding assays designed to map MUC13 interaction partners or assess glycosylation-dependent recognition events. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein meets the quality thresholds commonly required for cell-based functional assays and serves as a reliable positive control in immunoassays targeting MUC13 in cancer research contexts.

Target Background

Function

Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.

Gene References into Functions

The results suggest that MUC13 overexpression and loss of expression of AGR2 may predict the progression of Intraductal papillary mucinous neoplasm and an unfavorable prognosis in patients with Intraductal papillary mucinous neoplasm PMID: 29650332
our study first demonstrated that USF1 could activate the transcription of MUC13, thereby enhancing the proliferation and self-renewal of glioblastoma stem cells PMID: 29441861
MUC13 high expression is a novel independent adverse prognostic factor of clinical outcome in non-metastatic clear cell renal cell carcinoma patients after surgery. PMID: 27911274
Luciferase assays and western blot confirmed MUC13 as a target gene of miR1323p. PMID: 28339011
Results from this study demonstrate that MUC13 Functionally interacts and activates HER2 at p1248 in PDAC cells, leading to stimulation of HER2 signaling cascade, including ERK1/2, FAK, AKT and PAK1 as well as regulation of the growth, cytoskeleton remodeling and motility, invasion of PDAC cells-all collectively contributing to PDAC progression. PMID: 27321183
High MUC13 expression is associated with renal cell carcinoma and drug resistance. PMID: 28205224
A combination of MUC13/MUC20 expression was a potential prognostic marker for patients with ESCC, who received neoadjuvant chemotherapy followed by surgery. PMID: 26323930
Results show that pancreatic ductal adenocarcinoma cells express higher levels of MUC13 and is associated with poor outcome when expressed at low level. PMID: 25672256
Based on nasopharyngeal MUC13 gene expression in readily available Nasopharyngeal aspirate samples , we can discriminate between severity of disease in Respiratory syncytial virus infected infants. PMID: 25261323
These results suggest miR-145 as a novel regulator of MUC13 in pancreatic cancer. PMID: 25277192
Overexpression of MUC13 increased cell growth, colony formation, cell migration, and invasion in colon cancer cells. PMID: 24097071
MUC13 is epigenetically regulated in ovarian cancer. PMID: 25048476
Overexpression of MUC13 is associated with pancreatic neuroendocrine tumors. PMID: 24114056
GALNT14 may contribute to ovarian carcinogenesis through aberrant glycosylation of MUC13. PMID: 23708057
Metastatic colon cancer and liver metastasis tissue samples demonstrated significantly (p<0.05) higher cytoplasmic and nuclear MUC13 expression compared with non-metastatic colon cancer and adjacent normal colon samples. PMID: 22914648
MUC13 overexpression caused a significant increase in cell motility, invasion, proliferation, and anchorage-dependent or -independent clonogenicity while decreasing cell-cell and cell-substratum adhesion in pancreatic cancer. PMID: 22027689
The various functional domains of MUC13 may confer oncogenic potential to MUC13 PMID: 21450906
MUC13 is a good differentiation marker for gastrointestinal mucosa PMID: 15904467
Aberrant intestinal expression and allelic variant MUC13 is associated with inflammatory bowel disease. PMID: 17058067
Reflux laryngitis is associated with down-regulation of mucin gene expression. PMID: 18834073
Findings show the aberrant expression of MUC13 in ovarian cancer and that its expression alters the cellular characteristics of SKOV-3 cells. This implies a significant role of MUC13 in ovarian cancer. PMID: 19176398

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Apical cell membrane. Secreted. Note=Also exists as a soluble form.

Tissue Specificity

Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.

Database Links

HGNC: 7511

OMIM: 612181

KEGG: hsa:56667

STRING: 9606.ENSP00000312235

UniGene: Hs.5940

Code	CSB-YP887973HU
Abbreviation	Recombinant Human MUC13 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human MUC13 at 2 μg/ml can bind Anti-MUC13 recombinant antibody (CSB-RA887973MA1HU). The EC₅₀ is 2.592-2.923 ng/mL. Biological Activity Assay
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Recombinant Human Mucin-13(MUC13),partial (Active)

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