Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active)

Myosin regulatory light chain 12B plays a critical role in smooth muscle contraction and non-muscle cell motility by modulating myosin II activity through phosphorylation-dependent mechanisms. This full-length construct (aa 1–172) demonstrates functional folding, as confirmed by its ability to bind an anti-MYL9 recombinant antibody with an EC50 of 7.760–8.646 ng/mL in a functional ELISA, indicating that the C-terminal His tag does not disrupt the native epitope landscape. The quantified binding affinity provides a validated basis for protein-protein interaction assays investigating myosin light chain regulatory complexes and for antibody development workflows requiring a well-characterized positive control in Western blot or immunofluorescence experiments. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for cytoskeletal dynamics studies and structural biology applications, including crystallography or cryo-EM analysis of myosin regulatory assemblies.