MYL12B is a protein encoded by the myosin light chain 12B gene. Similar to MYL12A, it binds to the actin heavy chain to form an actin complex, jointly participating in processes such as cell motility regulation. Additionally, the phosphorylation state of MYL12B can affect the activity of the actin complex, thereby modulating muscle contraction strength and cell movement speed. In vitro studies have demonstrated that the diphosphorylated state of MYL12B results in enhanced MgATP enzyme activity and the formation of myosin filaments, which are crucial for the assembly of sarcomeric protein filaments. Overexpression of the diphosphorylated form of MYL12B leads to the formation of numerous sarcomeric protein bundles in transfected cells, whereas overexpression of the non-phosphorylated form results in a decrease in the number of sarcomeric protein bundles. This suggests that the phosphorylation state of MYL12B plays a pivotal role in the organization of sarcomeric protein filaments and contractile rings within cells. MYL12B plays a significant role in various diseases. Its phosphorylation level is associated with heart diseases such as myocardial injury and hypertrophy, as well as cancer. Consequently, MYL12B holds promise as a potential research target and biomarker for diseases like heart disease and cancer.
Recombinant Human Myosin regulatory light chain 12B(MYL12B) (Active)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MYL12B at 2μg/mL can bind Anti-MYL9 recombinant antibody (CSB-RA015318MA1HU),the EC50 is 7.760-8.646 ng/mL.
The following MYL12B reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
MYL12B Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA015308GA01HU | MYL12B Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA042133 | MYL12B Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA595808 | MYL12B Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
MYL12B Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP015308RA CSB-EP015308RA CSB-BP015308RA CSB-MP015308RA CSB-EP015308RA-B |
Recombinant Rat Myosin regulatory light chain 12B (Myl12b) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MYL12B Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP015308BO CSB-EP015308BO CSB-BP015308BO CSB-MP015308BO CSB-EP015308BO-B |
Recombinant Bovine Myosin regulatory light chain 12B (MYL12B) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MYL12B Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP015308HU CSB-BP015308HU CSB-MP015308HU CSB-EP015308HU-B |
Recombinant Human Myosin regulatory light chain 12B (MYL12B) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP015308HU | Recombinant Human Myosin regulatory light chain 12B (MYL12B) |
E.coli |
CSB-EP015308HUc7 | Recombinant Human Myosin regulatory light chain 12B(MYL12B) (Active) |
E.coli |
MYL12B Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP661006MO CSB-EP661006MO CSB-BP661006MO CSB-MP661006MO CSB-EP661006MO-B |
Recombinant Mouse Myosin regulatory light chain 12B (Myl12b) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |