Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PRLR at 2 μg/mL can bind Anti-PRLR recombinant antibody (CSB-RA018727A0HU), the EC50 is 126.8-171.9 ng/mL.
Alternative Names
PRLR; Prolactin receptor; PRL-R
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
25-234aa
Target Protein Sequence
QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO's recombinant human PRLR (25-234aa) is an active protein expressed in mammalian cells. It carries a 10xHis-tag at the C-terminus. Its purity is up to 95% measured by SDS-PAGE. Its endotoxin and activity have been accessed.
PRLR is expressed on mammary gland cells, pancreatic β-cells, adipocytes, and immune cells. Upon binding to the PRL, PRLR occurs dimerization, activating the JAK2-STAT5-SOCS, PI3K, and MAPK signaling pathways thus leading to increased survival, proliferation, and differentiation of these cells. PRLR is involved in reproduction, islet differentiation, regulation of fat stores, and immune responses. PRL-PRLR interactions are essential for the development and differentiation of the normal breast. Abnormal PRL-PRLR activity has been related to the development of numerous cancers, including breast cancer. Enhanced PRLR expression and high circulating concentrations of PRL have been related to an increased risk of tumor progression and invasion.
