Code | CSB-BP001262HU1 |
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Size | $352 |
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The recombinant human serine/threonine-protein kinase receptor R3 (ACVRL1) is an active protein generated in the baculovirus-infected insect cell expression system. The gene fragment encoding the 22-118aa of the human ACVRL1 protein was fused with the 6xHis-tag gene at the C-terminus and then cloned into the baculovirus transfer vector. The resulting ACVRL1 gene-containing vector was co-transfected into the cells with the baculovirus packaging mix to obtain the ACVRL1 gene-containing recombinant virus particles. Insect cells were infected with the recombinant bacmid, and transfected insect cells were selected through antibiotic screening and then cultured to harvest the recombinant human ACVRL1 protein. The purity of this protein is over 95% measured by SDS-PAGE. It contains low endotoxin, less than 1.0 EU/ug determined by the LAL method. Its activity was validated through a functional ELISA. Immobilized human CVRL1 at 2 μg/mL can bind the anti-ACVRL1 recombinant antibody (CSB-RA001262MA1HU), with the EC50 of 2.417-2.971 ng/mL.
ACVRL1, the endothelial cell-specific type I receptor of the TGF-β superfamily, is primarily expressed in vascular endothelial cells and plays a crucial role in the regulation of normal vascular development and remodeling, as well as pathological tumor angiogenesis. ACVRL1 controls a series of genes related to cancer cell survival, proliferation, angiogenesis, invasion, and metastasis, exerting a significant role in inhibiting the expression of key immune activation regulators and promoting the production of immune suppression factors. Therefore, targeting the ACVRL1 signaling pathway has become a promising strategy for anti-angiogenic cancer therapy. Developing active ACVRL1 proteins is of great significance in further studying its function and developing tumor drugs.
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