Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Activity

Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >7000 U/mg.

Target Names

ALPP

Uniprot No.

XP_045223825.1

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

21-504aa

Target Protein Sequence

IIPVEEENPDFWNRQAAEALGAAKKLQPIQTAAKNLIIFLGDGMGVSTVTAARILKGQKEEKLGPETPLAMDHFPYVALSKTYSVDKHVPDSAATATAYLCGVKGNFQTIGLSAAARYNQCNTTRGNEVVSVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDANMPGSARREGCKDIATQLISNMDIDVILGGGRKYMFRMGAPDPEYPHDYSQDGTRMDGKNLVQEWLAKHQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGAYPLRGSSIFGLAPGKAQDRKAYTALLYGNGPGYVLKDGARPDVTESESGSPEYRQQAAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPAGTTD

Mol. Weight

54.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Placental alkaline phosphatase serves as a biomarker in several malignancies and plays critical roles in phosphate metabolism during development, making the cynomolgus macaque ortholog valuable for translational studies bridging rodent models and human biology. This full-length mature protein (aa 21–504) demonstrates robust catalytic function with a specific activity exceeding 7000 U/mg against p-nitrophenylphosphate at pH 10.0 and 37°C, providing the quantitative performance required for kinetic parameter analysis including Km and Vmax determination, as well as inhibitor screening with accurate IC50 measurements. The C-terminal 10xHis tag preserves active site accessibility while enabling straightforward purification, and the >95% purity confirmed by SDS-PAGE supports its use as a positive control in enzyme-linked assays and for substrate specificity profiling where contaminating phosphatases would confound results. Endotoxin levels below 1.0 EU/μg align with standards expected in cell-based inhibition assays and drug candidate evaluation studies.

Code	CSB-MP4963MOV
Abbreviation	Recombinant Cynomolgus monkey ALPP protein (Active)
MSDS	MSDS Datasheet
Size	$148
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. The purity of ALPP was greater than 95% as determined by SEC-HPLC
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Recombinant Macaca fascicularis Alkaline phosphatase, placental type (ALPP) (Active)

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