Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ZG16B at 2 μg/mL can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC50 is 20.15-28.98 ng/mL.
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
23-169aa
Target Protein Sequence
GKMYGPGGGKYFTTTEDYDHEITGLRVSVGLLLVKSVQVKLGDTWDVKQGASGGNTQEVTLQPGEYITKVFVAFQTFLRGMVLYTSKDRTFYFGKLDGQIFSVYPSQEGQVLVGIYGQYGLLGIKSIGFEWNYPLEEPTTEPPVTVT
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Macaca fascicularis ZG16B recombinant protein was produced in Mammalian cell, where the gene sequence encoding Macaca fascicularis ZG16B (23-169aa) was expressed with the C-terminal 10xHis-tag. The purity of this ZG16B protein was greater than 90%. Its endotoxin content is less than 1.0 EU/ug as determined by the LAL method. The activity was validated. In a functional ELISA, immobilized Macaca fascicularis ZG16B at 2 μg/mL can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU) with the EC50 of 20.15-28.98 ng/mL.
ZG16B (zymogen granulin 16 homolog B), a pancreatic cancer up-regulated factor (PAUF), is known to bind carbohydrates and activate inner epithelial cells, angiogenesis and permeability. It is highly expressed in pancreatic cancer and can increase the adhesion between pancreatic cancer cells, promote angiogenesis and increase vascular permeability of pancreatic cancer by activating CXCR4, β-catenin, TPL2/MEK/ERK, FAK/Scr and other signaling pathways way to promote the migration, metastasis and progression of pancreatic cancer. At the same time, these signaling pathways are closely related to gemcitabine resistance. Therefore, we speculate that ZG16B is also involved in regulating the drug resistance of gemcitabine to pancreatic cancer cells. Therefore, in-depth research on it is beneficial to clarify the mechanism of pancreatic cancer chemotherapy resistance and provide new targets for the treatment of pancreatic cancer.
