Recombinant Cynomolgus monkey Transferrin receptor protein 1 (EGM_21146), partial (Active)

Transferrin receptor protein 1 mediates cellular iron uptake through endocytosis of iron-loaded transferrin, making it a critical target for studying iron homeostasis, erythropoiesis, and receptor-mediated endocytosis pathways in primate models. This cynomolgus macaque ortholog, spanning residues 89–760 and expressed in mammalian cells to preserve native glycosylation and disulfide architecture, demonstrates robust binding to an anti-TFRC recombinant antibody with an EC50 of 19.84–27.47 ng/mL in functional ELISA and a measured affinity constant of 0.28 nM by surface plasmon resonance. These quantitative binding parameters support its use in therapeutic antibody epitope mapping, competitive inhibition screening to identify blocking antibodies, and ligand-receptor interaction studies via ELISA, SPR, or biolayer interferometry platforms. The protein meets quality thresholds commonly required for drug discovery applications, with purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, providing a suitable basis for cell-based assays where low endotoxin is essential.