Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse CDH6 at 2 μg/mL can bind Anti-CDH6 recombinant antibody (CSB-RA005055MA1HU). The EC50 is 1.884-2.164 ng/mL.

Target Names

CDH6

Uniprot No.

P97326

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

19-615aa

Target Protein Sequence

TFSNPLSKRTSGFPAKRKALELSANSRNELSRSKRSWMWNQFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLDREEKPVYILRAQAVNRRTGRPVEPESEFIIKIHDINDNEPIFTKDVYTATVPEMADVGTFVVQVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADVGENAEIEYSITDGEGHEMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPHVEPRFLYLGPFKDSATVRIVVDDVDEPPVFSKLAYILQIREDARINTTIGSVAAQDPDAARNPVKYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKVLDVNDNAPEFAEFYETFVCEKAKADQLIQTLRAVDKDDPYSGHQFSFSLAPEAASSSNFTIQDNKDNTAGILTRKNGYNRHEMSTYLLPVVISDNDYPVQSSTGTVTVRVCACDHHGNMQSCHAEALIHPTGLSTGA

Mol. Weight

67.8 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Cadherin-6 mediates calcium-dependent homophilic cell–cell adhesion in neural and renal tissues, making it a critical target for studying developmental morphogenesis and tumor invasion pathways. This recombinant fragment spans residues 19–615, encompassing the extracellular cadherin repeats responsible for trans-interactions, and demonstrates quantifiable antibody binding with an EC50 of 1.884–2.164 ng/mL in functional ELISA, confirming proper epitope presentation. The mammalian expression system supports native glycosylation and disulfide bond formation, features that align with standards expected in cell adhesion and spreading assays where post-translational modifications influence cadherin clustering and avidity. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this protein provides a suitable basis for antibody development and validation workflows, as well as for dissecting cadherin-mediated signaling in tumor metastasis models where Cdh6 expression correlates with invasive phenotypes.

Target Background

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Gene References into Functions

Cadherin 6 expression in the nascent cortical plate may serve solidification of the protomap for cortical functional areas. PMID: 22875867
cadherin-6 plays essential roles in locomotor activity and ultrasonic vocalization PMID: 23173049
role of cadherin 6 as a novel ligand for alpha(IIb)beta(3) and highlights its function in thrombus formation. PMID: 22539596
Data show that during development, the adhesion molecule cadherin-6 is expressed by a subset of retinal ganglion cells and also by their targets in the brain. PMID: 21867880
Cadherin 6 is expressed in the floor and roof plates of the motor columns of the spinal cord. PMID: 12139922
identified a short 79-bp region that is implicated in regulating expression in cells once they have emigrated from the neural tube PMID: 18234175
Results describe the genetic control of Cdh6 expression by systematic integration of a reporter cassette into bacterial artificial chromosomes, with reporter activities evaluated in stable transgenic mouse lines. PMID: 18617008
These results suggest the role of Cdh6 in coordinating mouse hindbrain compartments possibly under the control of Hox gene regulatory network. PMID: 18948151

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Database Links

KEGG: mmu:12563

STRING: 10090.ENSMUSP00000037113

UniGene: Mm.458225

Code	CSB-MP005055MO1
Abbreviation	Recombinant Mouse CDH6 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse CDH6 at 2 μg/ml can bind Anti-CDH6 recombinant antibody (CSB-RA005055MA1HU). The EC₅₀ is 1.884-2.164 ng/mL. Biological Activity Assay
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Recombinant Mouse Cadherin-6 (Cdh6), partial (Active)

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