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Recombinant Mouse CUB domain-containing protein 1 (Cdcp1), partial (Active)

Recombinant Mouse CUB domain-containing protein 1 (Cdcp1), partial (Active)

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Code	CSB-MP719456MO
Abbreviation	Recombinant Mouse Cdcp1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse Cdcp1 at 2 μg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC₅₀ is 0.6397-0.8369 ng/mL. Biological Activity Assay
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Target Background

Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse Cdcp1 at 2 μg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC50 is 0.6397-0.8369 ng/ml.

Target Names

Cdcp1

Uniprot No.

Q5U462

Alternative Names

CUB domain-containing protein 1;Membrane glycoprotein gp140;Transmembrane and associated with src kinases;CD318;Cdcp1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

30-666aa

Target Protein Sequence

SEIALPQRSGVTVSIKLGNPALPVKICYIVMSRQHITELIIRPGERKSFTFSCSNPEKHFVLKIEKNIDCMSGPCPFGEVHLQPSTSELPILNRTFIWDVRAHKSIGLELQFATPRLRQIGPGESCADGVTHSISGHIDATEVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHRRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPGGFPEDELMTWQFVVPAHLRASVSFLNFNVSNCERKEERVEYYIPGSTTNPEVFRLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQRPQDESNKTYMVDLSRERTMSLTIEPRPVKHGRRFVPGCFVCLESRTCSTNVTLTAGSIHKISFLCDDLTRLWVNVEKTLSCLDHRYCYRQSFKLQVPDYILQLPVQLHDFSWKLLVPKDKLSLMLVPGQKLQQHTQERPCNTSFGYHVTSTTPGQDLYFGSFCSGGSIEKIQVKQNSSVTLRAYAPSFQQEVSKQGLIVSYTPYFKEEGIFTVTPDTKNKVYLRSPNWDRGLPALSSVSWNISVPSNQVACLTVLKERSGLACQSGRAFMIIQEQQSRAEEIFSLEEEVLPKPSFHHHSFWVNISNCSPMNGKQLDLLFWVTLTPRTVDL

Mol. Weight

73.2 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Cdcp1 plays a critical role in tumor cell adhesion and metastatic dissemination, making it a key target for understanding cancer progression mechanisms. This mammalian-expressed construct spans residues 30–666, preserving the native CUB domain architecture required for integrin interactions and downstream Src kinase signaling. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 0.64–0.84 ng/mL, confirming proper epitope presentation and supporting its use in antibody development workflows and immunoassay standardization. The protein meets the purity and endotoxin criteria typical for cell-based functional studies (>95% purity, <1.0 EU/μg), providing a suitable basis for cell adhesion and spreading assays, migration and invasion experiments, and tumor metastasis models where Cdcp1-mediated signaling influences cancer cell behavior.

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Target Background

Function

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.

Gene References into Functions

Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of autoimmune diseases. PMID: 28760953
Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells. PMID: 23243018
established mechanism for initiation of CDCP1-mediated signaling; demonstrated that processing of surface CDCP1 by extracellular serine proteases is prerequisite for CDCP1-dependent survival of cancer cells during metastasis; preventing cleavage of CDCP1 abrogated survival signaling associated with CDCP1 and induced PARP1 cleavage and PARP1-mediated apoptosis PMID: 22179830
CDCP1 may have a role in neoplasm metastasis; targeting it with a monoclonal antibody inhibits metastasis in a prostate cancer model PMID: 18483259

Subcellular Location

Cell membrane; Single-pass membrane protein.

Database Links

KEGG: mmu:109332

STRING: 10090.ENSMUSP00000042057

UniGene: Mm.27519

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