Recombinant Mouse CUB domain-containing protein 1 (Cdcp1), partial (Active)

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Code CSB-MP719456MO
Size $318
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Mouse Cdcp1 at 2 μg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC50 is 0.6397-0.8369 ng/mL. Biological Activity Assay
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Cdcp1 at 2 μg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC50 is 0.6397-0.8369 ng/ml.
Target Names
Cdcp1
Uniprot No.
Alternative Names
CUB domain-containing protein 1;Membrane glycoprotein gp140;Transmembrane and associated with src kinases;CD318;Cdcp1
Species
Mus musculus (Mouse)
Source
Mammalian cell
Expression Region
30-666aa
Target Protein Sequence
SEIALPQRSGVTVSIKLGNPALPVKICYIVMSRQHITELIIRPGERKSFTFSCSNPEKHFVLKIEKNIDCMSGPCPFGEVHLQPSTSELPILNRTFIWDVRAHKSIGLELQFATPRLRQIGPGESCADGVTHSISGHIDATEVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHRRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPGGFPEDELMTWQFVVPAHLRASVSFLNFNVSNCERKEERVEYYIPGSTTNPEVFRLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQRPQDESNKTYMVDLSRERTMSLTIEPRPVKHGRRFVPGCFVCLESRTCSTNVTLTAGSIHKISFLCDDLTRLWVNVEKTLSCLDHRYCYRQSFKLQVPDYILQLPVQLHDFSWKLLVPKDKLSLMLVPGQKLQQHTQERPCNTSFGYHVTSTTPGQDLYFGSFCSGGSIEKIQVKQNSSVTLRAYAPSFQQEVSKQGLIVSYTPYFKEEGIFTVTPDTKNKVYLRSPNWDRGLPALSSVSWNISVPSNQVACLTVLKERSGLACQSGRAFMIIQEQQSRAEEIFSLEEEVLPKPSFHHHSFWVNISNCSPMNGKQLDLLFWVTLTPRTVDL
Mol. Weight
73.2 kDa
Protein Length
Partial
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Recombinant mouse CDCP1 protein is generated using a mammalian cell expression system. The process involves fusing the gene segment encoding mouse CDCP1 (30-666aa) with a 10xHis-tag gene at the C-terminus and inserting it into a plasmid vector. The recombinant vector is then introduced into mammalian cells, which are cultured to allow protein expression. The resulting recombinant mouse CDCP1 protein exhibits purity above 95%, validated via SDS-PAGE, and endotoxin levels below 1.0 EU/ug, determined by the LAL method. Its binding activity is confirmed through assays with CDCP1 recombinant antibody (CSB-RA884474MA1HU), with the EC50 of 0.6397-0.8369 ng/ml.

CDCP1, a new human tumor-related gene reported in 2001, exhibits restricted expression in normal human tissues but is upregulated in most cancers, playing a critical role in tumor initiation, progression, and relapse. Clinical studies indicate a poor prognosis associated with CDCP1 in lung cancer, pancreatic cancer, renal cell carcinoma, ovarian cancer, and hepatocellular carcinoma. The signaling pathways regulated by CDCP1 play a significant role in promoting cancer and mediating resistance to chemotherapy and targeted therapy, making it a potential novel target for early cancer treatment. Furthermore, CDCP1-related antibodies display high affinity and specificity, underscoring CDCP1's suitability as a rational target for developing receptor-targeted drugs. Mouse CDCP1 shares approximately 88% homology with human CDCP1, suggesting similar structure and function. Currently, there is limited research on CDCP1-related drugs, and no clinical drugs are available. Thus, developing mouse CDCP1 protein can facilitate the screening of cross-recognizing clinical drugs targeting CDCP1 and enable animal model experiments.

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Target Background

Function
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Gene References into Functions
  1. Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of autoimmune diseases. PMID: 28760953
  2. Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells. PMID: 23243018
  3. established mechanism for initiation of CDCP1-mediated signaling; demonstrated that processing of surface CDCP1 by extracellular serine proteases is prerequisite for CDCP1-dependent survival of cancer cells during metastasis; preventing cleavage of CDCP1 abrogated survival signaling associated with CDCP1 and induced PARP1 cleavage and PARP1-mediated apoptosis PMID: 22179830
  4. CDCP1 may have a role in neoplasm metastasis; targeting it with a monoclonal antibody inhibits metastasis in a prostate cancer model PMID: 18483259
Subcellular Location
Cell membrane; Single-pass membrane protein.
Database Links
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