Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5 μg/mL can bind Anti-DSG3 recombinant antibody (CSB-RA007205A0HU), the EC₅₀ is 2.624-3.182 μg/mL.

Target Names

Dsg3

Uniprot No.

O35902

Research Area

Signal Transduction

Alternative Names

(Desmoglein-3)(130 kDa pemphigus vulgaris antigen homolog)

Molecular Characterization

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

50-617aa

Target Protein Sequence

EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP

Mol. Weight

66.0 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

A DNA fragment encoding the amino acids 50-617 of the mouse Dsg3 was expressed in the mammalian cells and tags indicated with 10xHis at the C-terminus. The product is the partial length recombinant mouse Dsg3 protein. Its purity reaches up to 85% determined by SDS-PAGE. On the gel, this Dsg3 protein showed a molecular weight of about 66.3 kDa. The LAL method determines that the endotoxin content of the Dsg3 protein is less than 1.0 EU/ug. This Dsg3 protein is also biologically active, and its activity has been validated through a functional ELISA. It can bind to the anti-Dsg3 antibody with the EC₅₀ of 2.624-3.182 μg/mL. And it is available now.

Dsg3, a member of the desmosomal cadherin family, is exclusively expressed in stratified epithelia. It has been identified as one of the autoantigens in the autoimmune blistering skin disease pemphigus Vulgaris (PV). Dsg3 participates in the keratinocyte cell-cell adhesion and the regulation of various signaling pathways involved in the progression and metastasis of cancer.

Target Background

Function

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Gene References into Functions

flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
Desmoglein 3 deficiency in mice leads to a phenotype characterized by cyclic alopecia in addition to the dramatic skin and mucocutaneous acantholysis observed in pemphigus patients. PMID: 25542773
Dsg3, via p38 MAPK, regulates the spatial distribution of the keratin filament network and stabilizes cell adhesion. PMID: 24782306
Squamous metaplasia after pulmonary epithelial injury may play a crucial role in redirecting the skin-specific autoimmune reaction to the lungs in paraneoplastic pemphigus (PNP). PMID: 23729442
Dsg3 deficiency results in perturbation of the desmosomal network at the transcriptional, translational, and interaction level, kinase activation, proteinase-mediated degradation, and hyper-adhesion. (Review) PMID: 23368972
Thus, Dsg3 does not display a clear function as a tumor suppressor in these mouse skin cancer models PMID: 23185521
IgG and IgM antibodies against desmoglein 3 have a pathogenic role in blister formation in pemphigus vulgaris PMID: 21718682
the adult passive transfer mouse model is ideally suited for detailed studies of Dsg3 antibody-mediated signaling in adult skin. PMID: 21956125
These data indicate a contribution of Dsg depletion to pemphigus vulgaris pathogenesis dependent on Ca(2+)-induced differentiation. PMID: 21864491
Aire has an important role in Dsg3 expression as well as in selection of T cells that help B cells to produce anti-Dsg3 IgG in thymus. PMID: 21048786
Loss of tolerance against Dsg3 in both B and T cells is important for the development of autoimmune state of pemphigus vulgaris. PMID: 11857336
Patchy hair loss in pemphigus vulgaris model mice as in Dsg3(-/-) mice. Ultrastructure of telogen hair follicles same as PV model mice and Dsg3(-/-) mice. Anti-Dsg3 autoantibodies interfere with cell-cell adhesion of keratinocytes in PV model mice. PMID: 12373337
perturbation of desmoglein 3 found in the Dsg3bal-Pas mice resulted in disadhesion of keratinocytes manifested with blistering phenotype. PMID: 12485423
anti-Dsg3 IgG antibodies can directly access Dsg3 present in desmosomes in vivo and cause the subsequent desmosome separation that leads to blister formation in pemphigus vulgaris PMID: 15140217
In pemphigus vulgaris antibodies were dominantly raised against middle to C-terminal extracellular domains of mouse Dsg3 where amino acid sequences are less conserved among desmoglein isoforms. Those antibodies may also be involved in blister formation. PMID: 15265525
importance of the companion layer, and particularly the Dsg1 and Dsg3 in this layer, in anchoring the anagen hair to the follicle. PMID: 15482466
Northern Blot analysis of wild-type and Plucked (pk) mutant mice indicates that expression of both desmoglein 1 (Dsg1) and desmoglein 3 (Dsg3) is up-regulated in the skin of mutant pk mice PMID: 15629430
skin-specific Dsg3 serves as an autoantigen in chronic skin inflammatory diseases accompanied by mast cell degranulation, including both murine systemic lupus erythematosus and other autoinflammatory diseases PMID: 16492738
Reduction of Dsg3 might be relevant to blister formation in pemphigus vulgaris. PMID: 17431647
distinct T cell responses could be switched after active immunization combined with different adjuvants, resulting in distinct anti-Dsg3 antibody isotypes with different pathogenic activities in Pemphigus disease development PMID: 18634826
Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice. PMID: 19525093

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.

Tissue Specificity

Expressed in epidermis.

Database Links

KEGG: mmu:13512

STRING: 10090.ENSMUSP00000064718

UniGene: Mm.106811

Code	CSB-MP007205MOd7
Abbreviation	Recombinant Mouse Dsg3 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5 μg/ml can bind Anti-DSG3 recombinant antibody (CSB-RA007205A0HU), the EC₅₀ is 2.624-3.182 μg/ml. Biological Activity Assay
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Recombinant Mouse Desmoglein-3 (Dsg3), partial (Active)

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Target Background

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